Reference : Increased expression of receptor activator of NF-kappa B ligand (RANKL), its receptor RA...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/25662
Increased expression of receptor activator of NF-kappa B ligand (RANKL), its receptor RANK and its decoy receptor osteoprotegerin in the colon of Crohn's disease patients
English
Franchimont, N. [> > > >]
Reenaers, Catherine [Université de Liège - ULg > Département des sciences cliniques > Rhumatologie >]
Lambert, Chantal mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
Belaiche, Jacques mailto [Université de Liège - ULg > Département des sciences cliniques > Hépato-gastroentérologie]
Bours, Vincent mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Génétique générale et humaine]
Malaise, Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Rhumatologie]
Delvenne, Philippe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Louis, Edouard mailto [Université de Liège - ULg > Département des sciences cliniques > Hépato-gastroentérologie >]
Dec-2004
Clinical & Experimental Immunology
Blackwell Publishing Ltd
138
3
491-498
Yes (verified by ORBi)
International
0009-9104
1365-2249
Oxford
United Kingdom
[en] Crohn's disease ; osteoporosis ; inflammation ; osteoprotegerin ; RANKL
Colon/immunology
[en] Colon/immunology ; Fluorescent Antibody Technique/methods ; Glycoproteins/blood/immunology ; Intestinal Mucosa/immunology
Membrane Glycoproteins/blood/immunology ; NF-kappa B/blood/immunology ; Osteoprotegerin ; RANK Ligand ; Receptor Activator of Nuclear Factor-kappa B ; Receptors, Cytoplasmic and Nuclear/blood/immunology ; Tissue Culture Techniques/methods
[en] Crohn's disease (CD) is associated with low bone mass due to chronic inflammation and other factors. Receptor activator of NF-kappaB ligand (RANKL), its receptor RANK and its decoy receptor osteoprotegerin (OPG) are potentially involved in this process as they regulate osteoclastogenesis and are influenced by pro-inflammatory cytokines. The aim of this study was to determine the levels of soluble RANKL (sRANKL), RANK and OPG expression both in the serum and in the colon of CD patients. Levels of sRANKL and OPG were assessed in the serum and the supernatants of cultured colonic biopsies in patients with CD and controls by ELISA. RANK expression was explored by immunostaining and immunofluorescence of fixed colonic samples. OPG and sRANKL levels were higher in the serum of CD patients as compared to age- and sex-matched controls. Levels of sRANKL and OPG were significantly enhanced in cultured colonic biopsies from CD, and OPG levels correlated with histological inflammation, and pro- and anti-inflammatory cytokine levels. No significant correlation was found for sRANKL. RANK(+) cells were increased in the colon of CD, particularly in inflamed areas. These cells were positive for CD68 or S100 protein. We conclude that serum and local levels of sRANKL and OPG are increased in CD. Moreover, RANK is expressed in the colonic mucosa by subpopulations of activated macrophages or dendritic cells at higher levels in CD compared to normal colon.
http://hdl.handle.net/2268/25662
also: http://hdl.handle.net/2268/56871
10.1111/j.1365-2249.2004.02643.x

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