|Reference : Radioimmunoassay of substance P in lung perfusate.|
|Scientific journals : Article|
|Human health sciences : Pharmacy, pharmacology & toxicology|
|Radioimmunoassay of substance P in lung perfusate.|
|Nemmar, A. [> > > >]|
|Gustin, Pascal [Université de Liège - ULg > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie >]|
|Delaunois, Annie [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]|
|Sulon, J. [> > > >]|
|Beckers, Jean-François [ > > ]|
|Journal of Pharmacological & Toxicological Methods|
|[en] Animals ; Capsaicin/pharmacology ; Lung/drug effects/metabolism ; Perfusion/methods ; Rabbits ; Radioimmunoassay/methods ; Reproducibility of Results ; Sensitivity and Specificity ; Substance P/pharmacokinetics ; Tyrosine/diagnostic use|
|[en] A radioimmunoassay of the undecapeptide substance P (SP) and its application in rabbit lung superfusate has been developed. The assay was based on the use of a radioactive tracer, 125I-SP-Tyr8, which showed, with an excess of antibodies, a specific binding greater than 90% and a nonspecific binding lower than 2%. This tracer was stable for 4 weeks at -20 degrees C. Its specific radioactivity was 384 Ci/mmol. The assay's lower limit of detection was 10 pg/ml (0.7 fmol). The determination of SP in rabbit lung perfusate required extraction and concentration using octadecylsilane cartridges. Under these conditions, the recovery of SP from experiments on lung perfusates (n=4) was 75.0+/-4.5%, and the intraassay and interassay coefficients of variation were 7.3+/-1.9% and 10.7+/-1.3%, respectively. Amounts of SP released in rabbit lung perfusates were determined following stimulation of pulmonary C fibers with capsaicin (10(-4) M). During the stimulation period, the values of SP from lung perfusates (n=6) increased significantly when compared with baseline values.|
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