[en] Bovine leukemia virus (BLV) p24 gene was expressed in Saccharomyces cerevisiae
under the control of the PHO5 (encoding repressible acid phosphatase, rAPase)
promoter. Yeast cells were transformed by a yeast-E. coli shuttle vector carrying
the PHO5 promoter, the p24 gene and the CYC1 transcription terminator. After low
inorganic phosphate (Pi) induction of the PHO5 promoter, p24 accumulated in the
producing cells up to a concentration representing 10% of total soluble proteins.
The expression level of p24 gene was not increased by insertion of the positive
regulatory gene PHO4 on the p24 expression vector. The p24 produced in this
system and incubated in crude yeast extract showed a remarkably high resistance
to proteolytic degradation, a feature that presumably correlates with the compact
globular conformation of the protein combined to the stabilizing effect of the
N-terminal residue.
Bruck, Mathot, Portetelle, Berte, Franssen, Herion, Burny (1982) Monoclonal antibodies define eight independent antigenic regions on the bovine leukemia virus (BLV) envelope glycoprotein gp51. Virology 122:342-352.
Bruck, Portetelle, Burny, Zavada (1982) Topographical analysis by monoclonal antibodies of BLV-gp51 epitopes involved in viral functions. Virology 122:353-362.
Bruck, Portetelle, Mammerickx, Mathot, Burny (1984) Epitopes of BLV glycoprotein gp51 recognized by sera of infected cattle and sheep. Leukemia Res. 8:315-321.
Bruck, Rensonnet, Portetelle, Cleuter, Mammerickx, Burny, Mamoun, Guillemain, Van Der Marten, Ghysdael (1984) Biologically active epitopes of bovine leukemia virus glycoprotein gp51: Their dependence on protein glycosylation and genetic variability. Virology 136:20-31.
Burny, Bruck, Chantrenne, Cleuter, Dekegel, Ghysdael, Kettmann, Leclerco, Leunen, Mammerickx, Portetelle (1980) Bovine leukemia virus: Molecular biology and epidemiology. Viral Oncology , G. Klein, Raven Press, New York; 231-298.
Burny, Bruck, Cleuter, Couez, Deschamps, Ghysdael, Gregoire, Kettmann, Mammerickx, Marbaix, Portetelle, Willems (1985) Bovine leukemia virus: A versatile agent with various pathogenic effects in various animal species. Cancer Res. (Suppl.) 45:4578-4582.
Burny, Cleuter, Dandoy, Kettmann, Mammerickx, Marbaix, Portetelle, Tartar, Van Den Broeke, Willems (1987) Leukemogenesis by bovine leukemia virus (BLV). Viruses and Human Cancer , R. Gallo, W. Haseltine, G. Klein, H. zur Hausen, A. R. Liss, New York; 345-353.
Deschamps, Kettmann, Burny (1981) Experiments with cloned complete tumor-derived bovine leukemia virus information prove that the virus is totally exogenous to its target animal species. J. Virol. 40:605-609.
Graves, Ferrer (1976) In vitro transmission and propagation of the bovine leukemia virus in monolayer cell cultures. Cancer Res. 36:4152-4159.
Jacobson, Raine, Mingioli, McFarlin (1988) Isolation of an HTLV-1 like retrovirus from patients with tropical spastic paraparesis. Nature (London) 331:540-543.
Mammerickx, Palm, Portetelle, Burny (1988) Experimental transmission of enzootic bovine leukosis to sheep: Latency period of the tumoral disease. Leukemia 2:103-107.
Maxam, Gilbert (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology , L. Grossman, K. Moldave, Academic Press, New York; 65:499-560.
Portetelle, Bruck, Mammerickx, Burny (1980) In animals infected by bovine leukemia virus (BLV) antibodies to envelope glycoprotein gp51 are directed against the carbohydrate moiety. Virology 105:223-233.
Portetelle, Bruck, Mammerickx, Burny (1983) Use of monoclonal antibody in an ELISA test for the detection of antibodies to bovine leukemia virus. J. Virol. Methods 6:19-29.
Rice, Stephens, Burny, Gilden (1985) The gag and pol genes of bovine leukemia virus: Nucleotide sequence and analysis. Virology 142:357-377.
Rice, Stephens, Couez, Deschamps, Kettmann, Burny, Gilden (1984) The nucleotide sequence of the env gene and post-env. region of bovine leukemia virus. Virology 138:82-93.
Sagata, Yasunaga, Tsuzuku-Kawanura, Ohishi, Ogawa (1985) Complete nucleotide sequence of the genome of bovine leukemia virus: Its evolutionary relationship to other retroviruses. Proc. Natl. Acad. Sci. USA 82:677-681.
Sanger, Coulson, Barrell, Smith, Roe (1980) Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J. Mol. Biol. 143:161-178.
Towbin, Staehelin, Gordon (1978) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets Procedure and some applications. Proceedings of the National Academy of Sciences 76:4350-4354.
Van Der Marten, Miller (1976) Replication of bovine leukemia virus in monolayer cell cultures. Bibl. Haematol. 43:360-362.
Wilson, Skehel, Wiley (1981) Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3Å resolution. Nature (London) 289:366-373.
Wiley, Wilson, Skehel (1981) Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation. Nature (London) 289:373-378.
