ORBi: Coulibaly Ibourahema - Determination of phospholipids extracted from Tsukamurella paurometabola C-924 after freeze-drying and during the subsequent storage.

Reference : Determination of phospholipids extracted from Tsukamurella paurometabola C-924 after fre...


	Document type :	Scientific congresses and symposiums : Poster
	Discipline(s) :	Life sciences : Biotechnology
	To cite this reference:	http://hdl.handle.net/2268/25316

Title :	Determination of phospholipids extracted from Tsukamurella paurometabola C-924 after freeze-drying and during the subsequent storage.
Language :	English
Author, co-author :	Coulibaly, Ibourahema [Université de Liège - ULg > > > Doct. sc. agro. & ingé. biol.]
	Yao, Amenan Anastasie [Université de Liège - ULg > > > Doct. sc. (bioch., bioch. mol.&cell., bioinf.&mod.-Bologne)]
	Dubois Dauphin, Robin [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Fauconnier, Marie-Laure [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Thonart, Philippe [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles >]
Publication date :	1-Apr-2009
Page number :	2x1
On invitation :	Yes
Audience :	International
Event name :	Biomedica 2009 -The Life Science Summit 1-2 April 2009
Event date :	du 1er avril 2009 au 2 avril 2009
Event organizer :	University of Liège
Event place (city) :	Liège
Event country :	Belgium
Keywords :	[en] Lactic acid bacteria ; Fatty acids ; phospholipids class separation ; evaporative light-scattering detection ; survival ; temperature
Abstract :	[en] The total lipids of Tsukamurella paurometabola C-924 were analysed after freeze-drying. Seven individual lipids classes were identified namly neutral lipids (NLs), fatty acids (FAs), phospholipids (PLs), sterol ester (SEs), triglycerides (TGs), diglycerides (DGs) and monoglycerides (MGs). The principal fatty acids identified in most lipid classes were palmitic (C16:0), palmitoleic (C16:1), oleic (C18:1), linoleic (C18:2), and linolenic (C18:3). PLs were the major constituents and accounted for 50-60% of the total lipids. PLs were fractionated. . PLs of Tsukamurella paurometabola content phosphatidic acid (PA), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), sphingomyelin (SM), lysophosphatidylcholine (LPC) and phosphatidylglycerol (PG). It was observed that PG had the highest proportion at most points relative to other PLs and was the predominant component of PLs (30%-56%). Evolution of individual rate was followed during stored at 20°C and 40°C with or without lithothamne400®, respectively.
Research units :	Unité de Bioindustries Gembloux Agro-Bio Tech
Permalink :	http://hdl.handle.net/2268/25316

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