ORBi: Yao Amenan Anastasie - Impact of polyunsaturated fatty acid degradation on survival and acidification activity of freeze-dried Weissella paramesenteroides LC11 during storage

Reference : Impact of polyunsaturated fatty acid degradation on survival and acidification activity ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biotechnology
	To cite this reference:	http://hdl.handle.net/2268/25302

Title :	Impact of polyunsaturated fatty acid degradation on survival and acidification activity of freeze-dried Weissella paramesenteroides LC11 during storage
Language :	English
Author, co-author :	Yao, Amenan Anastasie [Université de Liège - ULg > > > Doct. sc. (bioch., bioch. mol.&cell., bioinf.&mod.-Bologne)]
	Coulibaly, Ibourahema [Université de Liège - ULg > > > Doct. sc. agro. & ingé. biol.]
	Lognay, Georges [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Fauconnier, Marie-Laure [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Thonart, Philippe [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles >]
Publication date :	May-2008
Journal title :	Applied Microbiology & Biotechnology
Publisher :	Springer Verlag
Volume :	79
Issue/season :	6
Pages :	1045-1052
Audience :	International
ISSN :	0175-7598
e-ISSN :	1432-0614
City :	Berlin
Country :	Germany
Keywords :	[en] Lactic acid bacteria ; Lipid oxidation ; Oxylipins ; Polyinsaturated fatty acid ; Viability
Abstract :	[en] Abstract The impact of polyunsaturated fatty acid (PUFA) degradation on the survival and acidification activity of freeze-dried Weissella paramesenteroides LC11 was investigated over 90-days storage at 4 °C or 20 °C in vacuumsealed aluminium foil or glass tubes with two water activities (aw=0.11 or 0.23). Colony counts, acidification activity (% lactic acid/g), linoleic/palmitic (18:2/16:0) or linolenic/palmitic (18:3/16:0) ratio by gas chromatography and 18:2 or 18:3 oxylipins by reversed phase-high performance liquid chromatography were determined. The viable cells, acidification activity and 18:2/16:0 or 18:3/ 16:0 ratio decreased as the storage time increased. The survival, acidification activity and 18:2/16:0 or 18:3/16:0 ratio were greatest for the freeze-dried strain held in vacuum-sealed aluminium foil at 4 °C. The 18:2/16:0 or 18:3/16:0 ratio decrease was correlated with the accumulation of 18:2 or 18:3 oxylipins during storage in glass tubes. Hydroperoxy PUFAs, hydroxy PUFAs, divinyl ether PUFAs and oxo PUFAs were the main oxylipins identified. A large decrease in the 18:2/16:0 or 18:3/16:0 ratio and a rapid accumulation of oxylipins during storage might be enough to cause high cell death and loss of metabolic activity. These results provide further experimental support for the hypothesis that lipid oxidation and survival or activity of freeze-dried bacteria might be related.
Research units :	Wallon Center for industrial's biology
Funders :	University of liège, Gembloux Agro-Bio Tech
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/25302
	also: http://hdl.handle.net/2268/25493
DOI :	10.1007/s00253-008-1497-z
Commentary :	Amenan Yao and Ibourahema Coulibaly contribute to this work equally.

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