Reference : Detection and Identification of Pathotypes of Verocytotoxigenic Escherichia Coli Isolate...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/252
Detection and Identification of Pathotypes of Verocytotoxigenic Escherichia Coli Isolated from Weaned Piglets Using Gene Probes for Seven E. Coli Toxins
English
Mainil, Jacques mailto [Université de Liège - ULg > Maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
Daube, Georges mailto [Université de Liège - ULg > > Bactériologie et pathologie des maladies bactériennes >]
Deprez, Piet [CERVA-CODA > > > >]
Kaeckenbeeck, Albert [Université de Liège - ULg > > Bactériologie et pathologie des maladies bactériennes >]
Pohl, Pierre [CERVA-CODA > > > >]
1989
FEMS Microbiology Letters
50
3
345-349
Yes (verified by ORBi)
International
0378-1097
[en] E. coli ; VTEC ; Colony hybridization
[en] Seventy verocytotoxigenic (VTEC) and sixty-three non VTEC haemolytic Escherichia coli isolated from recently weaned piglets were examined by the colony hybridization assay using gene probes for three verocytotoxins: Edema disease principle (EDP) and Shiga-like toxins I and II (SLTI and SLTII). The results with the EDP and SLTII probes were identical. All VTEC hybridized with these two probes, while non VTEC did not. All 133 E. coli were negative for the SLTI probe. Hybridization of the plasmid content of 14 VTEC did not show any evidence for plasmid localization of the genes coding for the EDP. The 70 VTEC were also assayed with gene probes for heat-stable (STaP, STb) and heat-labile (LT, LTIIa) enterotoxins. Only the STb probe was hybridized by 36 of them. Most STb-positive isolates belonged to serotype O141: K85 biotypes 9 and 13 PC.
Researchers
http://hdl.handle.net/2268/252

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