Reference : Identification by two-dimensional electrophoresis of a new adhesin expressed by a low-pa...
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/25117
Identification by two-dimensional electrophoresis of a new adhesin expressed by a low-passaged strain of Mycoplasma bovis
English
Thomas, Anne [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes > >]
Leprince, Pierre mailto [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Dizier, Isabelle [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
Ball, Hywel [Veterinary Science Division > Bacteriology > > >]
Gevaert, Kris [Flanders Interuniversity Institute of Biotechnology > Department of Medical Protein Research > > >]
Van Damme, Jozef [Flanders Interuniversity Institute of Biotechnology > Department of Medical Protein Research > > >]
Mainil, Jacques mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
Linden, Annick mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Santé et pathologies de la faune sauvage >]
2005
Research in Microbiology
Elsevier
156
5-6, Jun-Jul
713-718
Yes (verified by ORBi)
International
0923-2508
Paris
France
[en] Mycoplasma bovis ; adhesion ; 2-D electrophoresis
[en] A significant decrease in adherence rates of Mycoplasma bovis to bovine bronchial epithelial (BBE) cells has been observed after passage of the organism in artificial medium. Analysis of the proteins expressed by M. bovis isolate 2610 by two-dimensional (2-D) electrophoresis demonstrated differences between the cells harvested after the 7th and 116th passage. Three silver-stained prominent spots observed in 2-D electrophoretic separation of protein extracts of the lower-passaged cells were considerably less strongly expressed in the sample from higher-passaged cells. These spots had a molecular mass of approximately 24 kDa and an isoelectric point of about 5. The mass spectrometry analysis of these trypsin-sensitive proteins led to their identification as a unique new member of the Vsps family of membrane-associated proteins. Serum from a mouse immunized with these proteins significantly reduced adherence of M. bovis to BBE cells. This result underlines the function of this new Vsp in adherence of M. bovis to host cells. (c) 2005 Elsevier SAS. All rights reserved.
Ministère Fédéral de l'Agriculture ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Wetenschappelijk Onderzoek - FWO ; COST
Researchers
http://hdl.handle.net/2268/25117
also: http://hdl.handle.net/2268/71061
10.1016/j.resmic.2005.02.008

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