Reference : Pathogenicity of pap-negative avian Escherichia coli isolated from septicaemic lesions
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Life sciences : Microbiology
http://hdl.handle.net/2268/25113
Pathogenicity of pap-negative avian Escherichia coli isolated from septicaemic lesions
English
Stordeur, P. [> > > >]
Bree, A. [> > > >]
Mainil, Jacques mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
Moulin-Schouleur, M. [> > > >]
Jun-2004
Microbes & Infection
Elsevier Science Bv
6
7
637-645
Yes (verified by ORBi)
International
1286-4579
Amsterdam
[en] colibacillosis ; poultry ; APEC ; Afa-VIII ; F17 adhesins
[en] Recent studies by DNA-DNA hybridisation assays conducted on a large collection of Escherichia coli strains isolated from chickens, ducks and turkeys suffering from colibacillosis, showed that 76% of the strains were negative for the presence of the pap gene cluster. The objective of this paper was to study the virulence associated with the avian E. coli strains negative for the P fimbriae, but carrying the f17 or the afa-8 gene cluster coding for adhesins associated with strains pathogenic for mammals. Three strains carrying the f17 fimbriae and three carrying the afa-8 adhesin-encoding gene cluster were studied in three in vivo experimental models of avian colibacillosis: subcutaneous inoculation of 1-day-old chicks, inoculation of specific-pathogen-free (SPF) chickens via the intra-thoracic air sac, and intra-tracheal inoculation of axenic chickens. The results showed that the six P-negative E. coli isolates carrying the f17 or the afa-8 gene cluster were lethal for 1-day-old chicks. They were also able to reproduce clinical signs and lesions of colibacillosis (aerosacculitis, pericarditis, perihepathitis), with bacteraemia and septicaemia, in SPF chickens inoculated via the thoracic air sacs as well as in axenic chickens inoculated by the intra-tracheal route. Further studies with f17 and afa-8 allelic mutants constructed by disruption must be performed to confirm a role of F17 fimbrial and Afa-VIII afimbrial adhesins in the pathogenesis of avian colibacillosis. (C) 2004 Elsevier SAS. All rights reserved.
http://hdl.handle.net/2268/25113
10.1016/j.micinf.2004.03.006

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
2004-Pathogenicity of pap-negative avian E coli isolated from septicaemic lesions.pdfPublisher postprint270.22 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.