Reference : Evaluation of immunogenicity and protective efficacy of a Microsporum canis metallopr...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/24992
Evaluation of immunogenicity and protective efficacy of a Microsporum canis metalloprotease subunit vaccine in guinea pigs.
English
Vermout, Sandy M mailto [> > > >]
Brouta, Frederic D [> > > >]
Descamps, Frederic F [> > > >]
Losson, Bertrand mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Mignon, Bernard mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
2004
FEMS Immunology & Medical Microbiology
Blackwell Publishing
40
1
75-80
Yes (verified by ORBi)
International
0928-8244
Oxford
United Kingdom
[en] Animals ; Antibodies, Fungal/blood ; Antigens, Fungal/immunology ; Dermatomycoses/prevention & control ; Disease Models, Animal ; Enzyme-Linked Immunosorbent Assay ; Female ; Fungal Vaccines/immunology ; Guinea Pigs ; Immunity, Cellular ; Metalloproteases/immunology ; Microsporum/enzymology/immunology ; Recombinant Proteins/immunology ; Treatment Outcome ; Vaccination ; Vaccines, Subunit/immunology
[en] In order to identify protective immunogens against Microsporum canis infection, a purified recombinant keratinolytic metalloprotease (r-MEP3) was tested as a subunit vaccine in experimentally infected guinea pigs. Both humoral and cellular specific immune responses developing towards r-MEP3 were evaluated, by enzyme-linked immunosorbent assay and by in vitro lymphocyte transformation tests respectively. Vaccination induced a strong antibody response, and a significant but transient lymphoproliferative response against the protein. However, the protocol failed to prevent fungal invasion or development of dermatophytic lesions. These results show that under the present experimental conditions, r-MEP3 specific antibodies are not protective against a challenge exposure. They also suggest that in the same model, the induction of cell-mediated immunity towards r-MEP3 is not sufficient, indicating the need for further research in the field of specific immune mechanisms involved in M. canis dermatophytosis.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/24992

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
2004-Evaluation of immunogenicity and protective efficacy.pdfPublisher postprint258.62 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.