Reference : Secreted subtilisins of Microsporum canis are involved in adherence of arthroconidia to ...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/24989
Secreted subtilisins of Microsporum canis are involved in adherence of arthroconidia to feline corneocytes.
English
Baldo, Aline mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Tabart, Jeremy [> > > >]
Vermout, Sandy [> > > >]
Mathy, Anne mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Collard, Alfred [> > > >]
Losson, Bertrand mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Mignon, Bernard mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
2008
Journal of Medical Microbiology
Lippincott Williams & Wilkins
57
Pt 9
1152-1156
Yes (verified by ORBi)
International
0022-2615
1473-5644
London
United Kingdom
[en] Animals ; Cats ; Cell Adhesion ; Cells, Cultured ; Fungal Proteins/metabolism ; Keratinocytes/microbiology ; Microsporum/enzymology/metabolism ; RNA, Messenger/metabolism ; Subtilisins/genetics/metabolism
[en] Microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis, mainly in cats and humans. The mechanisms involved in adherence of M. canis to epidermis have never been investigated. Here, a model was developed to study the adherence of M. canis to feline corneocytes through the use of a reconstructed interfollicular feline epidermis (RFE). In this model, adherence of arthroconidia to RFE was found to be time-dependent, starting at 2 h post-inoculation and still increasing at 6 h. Chymostatin, a serine protease inhibitor, inhibited M. canis adherence to RFE by 53%. Moreover, two mAbs against the keratinolytic protease subtilisin 3 (Sub3) inhibited M. canis adherence to RFE by 23%, suggesting that subtilisins, and Sub3 in particular, are involved in the adherence process.
http://hdl.handle.net/2268/24989
10.1099/jmm.0.47827-0

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