ORBi: Baldo Aline - Secreted subtilisins of Microsporum canis are involved in adherence of arthroconidia to feline corneocytes.

Reference : Secreted subtilisins of Microsporum canis are involved in adherence of arthroconidia to ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/24989

Title :	Secreted subtilisins of Microsporum canis are involved in adherence of arthroconidia to feline corneocytes.
Language :	English
Author, co-author :	Baldo, Aline [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
	Tabart, Jeremy [> > > >]
	Vermout, Sandy [> > > >]
	Mathy, Anne [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
	Collard, Alfred [> > > >]
	Losson, Bertrand [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
	Mignon, Bernard [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires >]
Publication date :	2008
Journal title :	Journal of Medical Microbiology
Publisher :	Lippincott Williams & Wilkins
Volume :	57
Issue/season :	Pt 9
Pages :	1152-1156
Audience :	International
ISSN :	0022-2615
e-ISSN :	1473-5644
City :	London
Country :	United Kingdom
Keywords :	[en] Animals ; Cats ; Cell Adhesion ; Cells, Cultured ; Fungal Proteins/metabolism ; Keratinocytes/microbiology ; Microsporum/enzymology/metabolism ; RNA, Messenger/metabolism ; Subtilisins/genetics/metabolism
Abstract :	[en] Microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis, mainly in cats and humans. The mechanisms involved in adherence of M. canis to epidermis have never been investigated. Here, a model was developed to study the adherence of M. canis to feline corneocytes through the use of a reconstructed interfollicular feline epidermis (RFE). In this model, adherence of arthroconidia to RFE was found to be time-dependent, starting at 2 h post-inoculation and still increasing at 6 h. Chymostatin, a serine protease inhibitor, inhibited M. canis adherence to RFE by 53%. Moreover, two mAbs against the keratinolytic protease subtilisin 3 (Sub3) inhibited M. canis adherence to RFE by 23%, suggesting that subtilisins, and Sub3 in particular, are involved in the adherence process.
Permalink :	http://hdl.handle.net/2268/24989
DOI :	10.1099/jmm.0.47827-0

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