Reference : Potential of Dried Egg Yolk as a Source of Immunoglobulins to Control Salmonella in t...
Scientific congresses and symposiums : Paper published in a book
Life sciences : Animal production & animal husbandry
http://hdl.handle.net/2268/24920
Potential of Dried Egg Yolk as a Source of Immunoglobulins to Control Salmonella in the Gastrointestinal Tract of the Broiler Chicken
English
Marcq, Christopher mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Portetelle, Daniel mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Chalghoumi, RAJA [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Thewis, André mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Beckers, Yves mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Aug-2009
17th European Symposium on Poultry Nutrition : Proceedings & Abstracts
181
Yes
No
International
17th European Symposium on Poultry Nutrition
23-27 August 2009
The World's Poultry Science Association, European Federation, UK Branch
Edinburgh
Scotland
[en] antibodies ; passive immunisation ; feed additive ; egg yolk powder ; Salmonella
[en] Egg yolk immunoglobulins (IgY) are considered as a promising tool of establishing passive immunity against enteric pathogens (e.g. Salmonella) in poultry (Kovacs-Nolan and Mine, 2005). In our previous works, in-feed incorporation of freeze-dried yolk powder (FYP) improved performances of Salmonella-infected broilers (Marcq et al., 2009). Nevertheless, no extra value could be attributed to the presence of specific IgY when a comparison was done between impact of hyperimmune or non immune FYP both concerning growth performances and Salmonella infection. We hypothesized that these inconclusive in vivo results were related to the sensitivity of IgY to gastrointestinal conditions.

Egg yolks with high IgY titers directed against Salmonella Enteritidis and Typhimurium were obtained from laying hens immunized as previously described (Chalghoumi et al., 2008). Three feed additives derived from these eggs were produced: FYP, spray-dried yolk powder (SYP), and freeze-dried water-soluble fraction of yolk powder (WSFP). A force-feeding assay was realized on these additives with 48 five-week-old males Ross broiler chicken (Salmonella spp.-free status). Survival of undigested IgY and bioactive Fab fragments was evaluated in the intestinal tract by ELISA. Statistical analyses were done using GLM procedure of Minitab 15.1. Software. Differences were considered significant when P<0.05.

As a result of the digestive process, the levels of total and specific undigested IgY found throughout the intestine were dramatically reduced for the three additives. For FYP, no difference was observed with intestinal contents of control animals (P>0.05) and for WSFP, which correspond to semi-purified IgY, the immunological activity reached the same level than that for SYP (P>0.05) whereas its initial level was almost ten times higher. The results suggest a protective effect of whole yolk on IgY with SYP but such effect was not observed with FYP. High levels of Fab fragments were identified in intestinal contents, highlighting the importance of protein digestion in the digestive tract and especially pepsin digestion. These Fab fragments conserve their immunological functionality and remained detectable in all intestinal segments including the caecum which represents the major site of Salmonella infection in poultry.

These results can explain our previous challenge data and reveal that a protection treatment should be applied to dried egg yolk to improve the resistance of IgY to gastrointestinal conditions in broilers.
Région wallonne : Direction générale de l'Agriculture - DGA
D31-1191 : Développement et utilisation des anticorps du jaune d’œuf en vue du contrôle des Salmonella spp. dans le tube digestif du poulet de chair
Researchers ; Professionals
http://hdl.handle.net/2268/24920

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