Paper published in a book (Scientific congresses and symposiums)
Potential of Dried Egg Yolk as a Source of Immunoglobulins to Control Salmonella in the Gastrointestinal Tract of the Broiler Chicken
Marcq, Christopher; Portetelle, Daniel; Chalghoumi, RAJA et al.
2009In 17th European Symposium on Poultry Nutrition : Proceedings & Abstracts
Peer reviewed
 

Files


Full Text
ESPN2009_ShortCommunication.pdf
Publisher postprint (479.55 kB)
Communication orale présentée
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
antibodies; passive immunisation; feed additive; egg yolk powder; Salmonella
Abstract :
[en] Egg yolk immunoglobulins (IgY) are considered as a promising tool of establishing passive immunity against enteric pathogens (e.g. Salmonella) in poultry (Kovacs-Nolan and Mine, 2005). In our previous works, in-feed incorporation of freeze-dried yolk powder (FYP) improved performances of Salmonella-infected broilers (Marcq et al., 2009). Nevertheless, no extra value could be attributed to the presence of specific IgY when a comparison was done between impact of hyperimmune or non immune FYP both concerning growth performances and Salmonella infection. We hypothesized that these inconclusive in vivo results were related to the sensitivity of IgY to gastrointestinal conditions. Egg yolks with high IgY titers directed against Salmonella Enteritidis and Typhimurium were obtained from laying hens immunized as previously described (Chalghoumi et al., 2008). Three feed additives derived from these eggs were produced: FYP, spray-dried yolk powder (SYP), and freeze-dried water-soluble fraction of yolk powder (WSFP). A force-feeding assay was realized on these additives with 48 five-week-old males Ross broiler chicken (Salmonella spp.-free status). Survival of undigested IgY and bioactive Fab fragments was evaluated in the intestinal tract by ELISA. Statistical analyses were done using GLM procedure of Minitab 15.1. Software. Differences were considered significant when P<0.05. As a result of the digestive process, the levels of total and specific undigested IgY found throughout the intestine were dramatically reduced for the three additives. For FYP, no difference was observed with intestinal contents of control animals (P>0.05) and for WSFP, which correspond to semi-purified IgY, the immunological activity reached the same level than that for SYP (P>0.05) whereas its initial level was almost ten times higher. The results suggest a protective effect of whole yolk on IgY with SYP but such effect was not observed with FYP. High levels of Fab fragments were identified in intestinal contents, highlighting the importance of protein digestion in the digestive tract and especially pepsin digestion. These Fab fragments conserve their immunological functionality and remained detectable in all intestinal segments including the caecum which represents the major site of Salmonella infection in poultry. These results can explain our previous challenge data and reveal that a protection treatment should be applied to dried egg yolk to improve the resistance of IgY to gastrointestinal conditions in broilers.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Marcq, Christopher ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Portetelle, Daniel ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Chalghoumi, RAJA ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Thewis, André ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Beckers, Yves  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Language :
English
Title :
Potential of Dried Egg Yolk as a Source of Immunoglobulins to Control Salmonella in the Gastrointestinal Tract of the Broiler Chicken
Publication date :
August 2009
Event name :
17th European Symposium on Poultry Nutrition
Event organizer :
The World's Poultry Science Association, European Federation, UK Branch
Event place :
Edinburgh, United Kingdom
Event date :
23-27 August 2009
Audience :
International
Main work title :
17th European Symposium on Poultry Nutrition : Proceedings & Abstracts
Pages :
181
Peer reviewed :
Peer reviewed
Name of the research project :
D31-1191 : Développement et utilisation des anticorps du jaune d’œuf en vue du contrôle des Salmonella spp. dans le tube digestif du poulet de chair
Funders :
DGA - Région wallonne. Direction générale de l'Agriculture [BE]
Available on ORBi :
since 03 October 2009

Statistics


Number of views
167 (19 by ULiège)
Number of downloads
78 (9 by ULiège)

Bibliography


Similar publications



Contact ORBi