Reference : Osmotic behaviour of in vitro produced bovine blastocysts in cryoprotectant solutions as...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/24519
Osmotic behaviour of in vitro produced bovine blastocysts in cryoprotectant solutions as a potential predictive test of survival
English
Kaidi, Safia [ > > ]
Donnay, I. [ > > ]
Lambert, Philippe mailto [Université de Liège - ULg > Institut des sciences humaines et sociales > Méthodes quantitatives en sciences sociales >]
Dessy, F. [ > > ]
Massip, P. [ > > ]
2000
Cryobiology
Academic Press
41
106-115
Yes (verified by ORBi)
International
0011-2240
1090-2392
New York
NY
[en] in vitro bovine embryo ; cryoprotectant ; osmotic behavior
[en] The osmotic behavior of bovine blastocysts produced in vitro was filmed during exposure to and dilution of cryoprotectant solutions used for vitrification. The relationship between the changes in the diameter of embryos and their subsequent survival was assessed. Embryos collected on Day 6 and Day 7 postinsemination were exposed to 10% glycerol (GLY) for 5 min, 10% GLY + 20% ethylene glycol (EG) for 5 min, and 25% Gly + 25% EG for 30 s, before dilution in 0.85 M galactose and finally in embryo transfer freezing medium (ETF). Embryos that had a higher probability of survival behaved as perfect osmometers, shrinking, reexpanding, or swelling according to an identical pattern, whereas embryos that deviated from this standard usually did not survive. The initial embryo diameter, duration of shrinkage and expansion in 10% glycerol, duration of reexpansion in ETF, and final embryo diameter were clearly predictive of the ability to hatch after culture in vitro. On a given day postinsemination, larger blastocysts were more likely than smaller blastocysts to survive and hatch after exposure to cryoprotectants with or without vitrification.
http://hdl.handle.net/2268/24519
10.1006/cryo.2000.2272
http://www.sciencedirect.com/science/article/B6WD5-45FCMY4-P/2/468264834e408807aa4adb9210bf1508

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