in vitro bovine embryo; cryoprotectant; osmotic behavior
Abstract :
[en] The osmotic behavior of bovine blastocysts produced in vitro was filmed during exposure to and dilution of cryoprotectant solutions used for vitrification. The relationship between the changes in the diameter of embryos and their subsequent survival was assessed. Embryos collected on Day 6 and Day 7 postinsemination were exposed to 10% glycerol (GLY) for 5 min, 10% GLY + 20% ethylene glycol (EG) for 5 min, and 25% Gly + 25% EG for 30 s, before dilution in 0.85 M galactose and finally in embryo transfer freezing medium (ETF). Embryos that had a higher probability of survival behaved as perfect osmometers, shrinking, reexpanding, or swelling according to an identical pattern, whereas embryos that deviated from this standard usually did not survive. The initial embryo diameter, duration of shrinkage and expansion in 10% glycerol, duration of reexpansion in ETF, and final embryo diameter were clearly predictive of the ability to hatch after culture in vitro. On a given day postinsemination, larger blastocysts were more likely than smaller blastocysts to survive and hatch after exposure to cryoprotectants with or without vitrification.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Kaidi, Safia
Donnay, I.
Lambert, Philippe ; Université de Liège - ULiège > Institut des sciences humaines et sociales > Méthodes quantitatives en sciences sociales
Dessy, F.
Massip, P.
Language :
English
Title :
Osmotic behaviour of in vitro produced bovine blastocysts in cryoprotectant solutions as a potential predictive test of survival
Publication date :
2000
Journal title :
Cryobiology
ISSN :
0011-2240
eISSN :
1090-2392
Publisher :
Academic Press, New York, United States - New York
Carvalho R.V., Del Campo M.R., Plante Y., Mapletoft R.J. (1995) Effects of stage development on sex ratio and survival after freezing of day 7 bovine IVF embryos. Theriogenology 39:183.
Cox D.R., Snell E.J. Analysis of Binary Data, Chapman and Hall, London; 1970.
Dagnelie P. (1975) Les methodes de l'inference statistique., 'Theorie et Methodes statistiques.' Gembloux Agronomic Press, Gembloux Belgium; 2:213-240.
Dinnyes A., Carolan C., Lonergan P., Solti L., Massip A., Mermillod P. (1995) In vitro survival of in vitro produced (IVP) bovine embryos frozen or vitrified by techniques suitable for direct transfer. Theriogenology 43:179.
Dinnyes A., Lonergan P., Fair T., Boland M., Yang X. (1999) Timing of the first cleavage post-insemination affects cryosurvival of in vitro produced bovine blastocysts. Mol. Reprod. Dev. 53:318-324.
Dobrinsky J.R. (1996) Cellular approach to cryopreservation of embryos. Theriogenology 45:17-26.
Donnay I., Van Langendonckt A., Auquier P., Massip A., Dessy F. (1996) A new phosphate-free simple medium for culture and co-culture of cattle embryos. J. Reprod. Fertil. 18:97.
Donnay I., Auquier P., Kaidi S., Carolan C., Lonergan P., Mermillod P., Massip A. (1998) Vitrification of in vitro produced bovine blastocysts: Methodological studies and developmental capacity. Anim. Reprod. Sci. 52:93-104.
Greve T., Avery B., Callesen H. (1993) Viability of in vivo and in vitro produced bovine embryos. Reprod. Domest. Anim. 28:164-169.
Han Y.M., Yamashina H., Koyama N., Lee K.K., Fukui Y. (1994) Effects of quality and developmental stage on the survival of IVF derived bovine blastocysts cultured in vitro after freezing and thawing. Theriogenology 42:645-654.
Hasler J.F., Hurtgen P.J., Jin Z.Q., Stokes J.E. (1996) Survival of IVF derived bovine embryos frozen in glycerol or ethylen glycol. Theriogenology 48:563-579.
Hoffman L.D., Bradley G.L. (1992) Double integrals., 'Calculus for Business, Economics, and Social and Life Sciences,' 5th ed., McGraw-Hill, New York; .
Hotamisligil S., Toner M., Powers R.D. (1996) Changes in membrane integrity, cytoskeletal structure, and developmental potential of murine oocytes after vitrification in ethylene glycol. Biol. Reprod. 55:161-168.
Jiang H.S., Wang W.L., Lu K.H., Gordon I., Polge C. (1998) Examination of cell numbers of blastocysts derived from IVM, IVF and IVC of bovine follicular oocytes. Theriogenology 37:229.
Jackowski S.C., Leibo S.P., Mazur P. (1980) Glycerol permeabilities of fertilized and unfertilized mouse ova. J. Exp. Zool. 212:329-341.
Kaidi S., Donnay I., Van Langendonckt A., Dessy F., Massip A. (1998) Comparison of two co-culture systems to assess the survival of in vitro produced bovine blastocysts after vitrification. Anim. Reprod. Sci. 52:39-50.
Kaidi S., Van Langendonckt A., Massip A., Dessy F., Donnay I. (1999) Cellular alteration after dilution of cryoprotective solutions used for vitrification of in vitro produced bovine embryos. Theriogenology 52:515-525.
Kasai M., Niwa K., Iritani A. (1981) Effects of various cryoprotective agents on the survival of unfrozen and frozen mouse embryos. J. Reprod. Fertil. 63:175-180.
Kuwayama M., Fujikawa S., Nagai T. (1994) Ultrastructure of IVM-IVF bovine blastocysts vitrified after equilibration in gly 1,2-propanediol using 2-step and 16-step procedures. Cryobiology 31:415-422.
Leibo S.P. (1980) Water permeability and its activation energy of fertilized and unfertilized mouse ova. J. Membr. Biol. 53:179-188.
Leibo S.P. (1983) A one-step in situ dilution method for frozen-thawed bovine embryos. Cryo-Letters 4:387-400.
Leibo S.P. (1986) Cryobiology: Preservation of mammalian embryo., 'Genetic Engineering of Animals: An Agricultural Perspective' (J. W. Evans and A. Hollaender, Eds.). Plenum, New York; 251-272.
Leibo S.P., Loskutoff N.M. (1993) Cryobiology of in vitro-derived bovine embryos. Theriogenology 39:81-94.
Mahmoudzadeh A.R., Van Soom A., Ysebaert M.T., De Kruif A. (1994) Comparison of two-step vitrification versus controlled freezing on survival of in vitro-produced cattle embryos. Theriogenology 42:1389-1397.
Mahmoudzadeh A.R., Van Soom A., Bols P., Ysebaert M.T., De Kruif A. (1995) Optimization of a simple vitrification procedure for bovine embyos produced in vitro: Effect of developmental stage, two step addition of cryoprotectant and sucrose dilution on embryonic survival. J. Reprod. Fertil. 103:33-39.
Massip A., Mermillod P., Dinnyes A. (1995) Morphology and biochemistry of in vitro produced bovine embryos: Implications for their cryopreservation. Hum. Reprod. 10:3004-3011.
Mazur P. (1976) Freezing and low temperature storage of living cells., 'Basic Aspects of Freeze Preservation of Mouse strains' (O. Muhlbock, Ed.). Fischer, Stuttgart; 1-12.
Mazur P., Schneider U. (1986) Osmotic responses of preimplantation mouse and bovine embryos and their cryobiological implications. Cell. Biophys. 8:259-284.
Paynter S.J., Cooper A., Gregory L., Fuller B.J., Shaw R.W. (1999) Permeability characteristics of human oocytes in the presence of the cryoprotectant dimethylsulphoxide. Hum. Reprod. 14:2338-2342.
Pfaff R.T., Liu G., Gao D., Peter A.T., Li T.K., Critser J.K. (1998) Water and DMSO membrane permeability characteristics of in vivo and in vitro derived and cultured murine oocytes and embryos. Mol. Hum. Reprod. 4:51-59.
Plante L., King W.A. (1994) Light and electron microscopic analysis of bovine embryos derived by in vitro and in vivo fertilisation. J. Assist. Reprod. Genet. 11:515-529.
Prather R.S., First N.L. (1993) Cell-to-cell coupling in early stage bovine embryo: A preliminary report. Theriogenology 39:561-567.
Rall W.F. (1987) Factors affecting the survival of mouse embryos cryopreserved by vitrification. Cryobiology 24:387-402.
Saha S., Suzuki T. (1997) Vitrification of in vitro produced bovine embryos at different ages using one- and three-step addition of cryoprotective additives. Reprod. Fertil. Dev. 9:741-746.
Schneider U., Mazur P. (1984) Osmotic consequences of cryoprotectant permeability and its relation to the survival of frozen-thawed embryos. Theriogenology 21:68-79.
Szell A., Shelton J.N., Szell K. (1989) Osmotic characteristics of sheep and cattle embryos. Cryobiology 26:297-301.
Thompson J.G. (1997) Comparison between in vivo-derived and in vitro-produced pre-elongation embryos from domestic ruminants. Reprod. Fertil. Dev. 9:341-354.