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ISOLATION OF SUCROSE SYNTHASE FROM RICE (ORYZA-SATIVA) GRAINS IN PILOT-SCALE FOR APPLICATION IN CARBOHYDRATE SYNTHESIS
ELLING, Lothar; GULDENBERG, Birgit; GROTHUS, Marita et al.
1995In Biotechnology and Applied Biochemistry, 21 (Part 1), p. 29-37
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Keywords :
sucrose synthase; purification; rice (Oryza sativa); nucleotide sugar; saccharide synthesis
Abstract :
[en] The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the 9 kg scale of rice (Oryza sativa) grains was achieved in five steps with 11.3% yield and 192-fold purification. The final sucrose synthase preparation was free of nucleotide di- and monophosphatases, but did contain 0.05% of both invertase and UDP-glucose-hydrolysing activity. It can be efficiently utilized for the synthesis of activated sugars. Its application for the synthesis of saccharides is possible taking into account that 15% UDP-glucose is hydrolysed within 15 h incubation time
Disciplines :
Biotechnology
Author, co-author :
ELLING, Lothar
GULDENBERG, Birgit
GROTHUS, Marita
Zervosen, Astrid 
PEUS, Monika
HELFER, Anke
STEIN, Andreas
ADRIAN, Holger
KULA, Maria-Regina
Language :
English
Title :
ISOLATION OF SUCROSE SYNTHASE FROM RICE (ORYZA-SATIVA) GRAINS IN PILOT-SCALE FOR APPLICATION IN CARBOHYDRATE SYNTHESIS
Publication date :
1995
Journal title :
Biotechnology and Applied Biochemistry
ISSN :
0885-4513
eISSN :
1470-8744
Publisher :
Portland Press, London, United Kingdom
Volume :
21
Issue :
Part 1
Pages :
29-37
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 29 September 2009

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