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| Reference : ISOLATION OF SUCROSE SYNTHASE FROM RICE (ORYZA-SATIVA) GRAINS IN PILOT-SCALE FOR APPLICA... |
| Scientific journals : Article | |||
| Life sciences : Biotechnology | |||
| http://hdl.handle.net/2268/24396 | |||
| ISOLATION OF SUCROSE SYNTHASE FROM RICE (ORYZA-SATIVA) GRAINS IN PILOT-SCALE FOR APPLICATION IN CARBOHYDRATE SYNTHESIS | |
| English | |
| ELLING, Lothar [> > > >] | |
| GULDENBERG, Birgit [> > > >] | |
| GROTHUS, Marita [> > > >] | |
Zervosen, Astrid  [> > > >] | |
| PEUS, Monika [> > > >] | |
| HELFER, Anke [> > > >] | |
| STEIN, Andreas [> > > >] | |
| ADRIAN, Holger [> > > >] | |
| KULA, Maria-Regina [> > > >] | |
| 1995 | |
| Biotechnology & Applied Biochemistry | |
| Portland Press | |
| 21 | |
| Part 1 | |
| 29-37 | |
| International | |
| 0885-4513 | |
| 1470-8744 | |
| London | |
| United Kingdom | |
| [en] sucrose synthase ; purification ; rice (Oryza sativa) ; nucleotide sugar ; saccharide synthesis | |
| [en] The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the 9 kg scale of rice (Oryza sativa) grains was achieved in five steps with 11.3% yield and 192-fold purification. The final sucrose synthase preparation was free of nucleotide di- and monophosphatases, but did contain 0.05% of both invertase and UDP-glucose-hydrolysing activity. It can be efficiently utilized for the synthesis of activated sugars. Its application for the synthesis of saccharides is possible taking into account that 15% UDP-glucose is hydrolysed within 15 h incubation time | |
| Researchers ; Professionals | |
| http://hdl.handle.net/2268/24396 |
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