Reference : ISOLATION OF SUCROSE SYNTHASE FROM RICE (ORYZA-SATIVA) GRAINS IN PILOT-SCALE FOR APPL...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/24396
ISOLATION OF SUCROSE SYNTHASE FROM RICE (ORYZA-SATIVA) GRAINS IN PILOT-SCALE FOR APPLICATION IN CARBOHYDRATE SYNTHESIS
English
ELLING, Lothar [> > > >]
GULDENBERG, Birgit [> > > >]
GROTHUS, Marita [> > > >]
Zervosen, Astrid mailto [> > > >]
PEUS, Monika [> > > >]
HELFER, Anke [> > > >]
STEIN, Andreas [> > > >]
ADRIAN, Holger [> > > >]
KULA, Maria-Regina [> > > >]
1995
Biotechnology & Applied Biochemistry
Portland Press
21
Part 1
29-37
Yes (verified by ORBi)
International
0885-4513
1470-8744
London
United Kingdom
[en] sucrose synthase ; purification ; rice (Oryza sativa) ; nucleotide sugar ; saccharide synthesis
[en] The application of sucrose synthase as a valuable biocatalyst for the synthesis of activated sugars and saccharides requires large amounts of enzyme. The purification of sucrose synthase starting from the 9 kg scale of rice (Oryza sativa) grains was achieved in five steps with 11.3% yield and 192-fold purification. The final sucrose synthase preparation was free of nucleotide di- and monophosphatases, but did contain 0.05% of both invertase and UDP-glucose-hydrolysing activity. It can be efficiently utilized for the synthesis of activated sugars. Its application for the synthesis of saccharides is possible taking into account that 15% UDP-glucose is hydrolysed within 15 h incubation time
Researchers ; Professionals
http://hdl.handle.net/2268/24396

There is no file associated with this reference.

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.