Article (Scientific journals)
Absence of Laminin Deposition in Breast Cancer and Metastases except to the Brain
Castronovo, Vincenzo; Bracke, M. E.; Mareel, M. M. et al.
1991In Pathology - Research and Practice, 187 (2-3), p. 201-8
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Keywords :
laminin; invasion; MO4 cells; Human brain metastasis; in vitro invasion model
Abstract :
[en] Laminin, a major glycoprotein of basement membrane has been found to play significant roles during invasion and metastases. In this study, we have examined the distribution of laminin in several human brain carcinoma metastases, human breast cancers, skin and lymph node metastases of breast cancer as well as in an in vitro and an in vivo model of invasion. A laminin accumulation was demonstrated a) at the border between human metastatic carcinoma cells and surrounding neural tissue; b) at the invasive edge between MO4 cells (a highly malignant cell line which synthesizes large amounts of laminin) and host tissues of syngenic mice; c) at the front of invasion between MO4 cells and precultured heart fragments in an in vitro model of invasion. Laminin, but not type IV collagen, promoted attachment of MO4 cells. This attachment was inhibited by preincubation of laminin matrix support with (+)-catechin, a flavonoid which also prevented invasion of the precultured heart fragment in vitro. Our data demonstrate that laminin accumulates between malignant cells and host tissue in human brain metastases and in an in vitro and an in vivo model of invasion. In these later models, accumulation of laminin is the consequence, at least in part, of its biosynthesis by MO4 cells. Since laminin promotes attachment of malignant cells in vitro, increases invasiveness and metastatic activities of murine malignant cells, it is tempting to speculate that laminin synthesized by invasive cells and accumulated at the front of invasion plays a significant role in the first step of invasion.
Disciplines :
Biochemistry, biophysics & molecular biology
General & internal medicine
Author, co-author :
Castronovo, Vincenzo ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire
Bracke, M. E.
Mareel, M. M.
Reznik, Michel ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Language :
English
Title :
Absence of Laminin Deposition in Breast Cancer and Metastases except to the Brain
Publication date :
March 1991
Journal title :
Pathology - Research and Practice
ISSN :
0344-0338
eISSN :
1618-0631
Publisher :
Elsevier, Netherlands
Volume :
187
Issue :
2-3
Pages :
201-8
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
CGER pour Cancer Research
Fondation contre le Cancer [BE]
Oeuvre Belge du Cancer
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