Reference : Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/24130
Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.
English
[fr] Caractérisation des protéines codées par l'opéron yoxA-dacC de Bacillus subtilis
Duez, Colette mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Zervosen, Astrid mailto [Université de Liège - ULg > > Centre de recherches du cyclotron >]
Teller, Nathalie mailto [Université de Liège - ULG > > > > Centre de recherche du cyclotron > >]
Melkonian, Rémy mailto [Université de Liège - ULG > > > > Centre d'ingénierie des protéines > >]
Banzubazé, Emmanuel [ > > ]
Bouillenne, Fabrice mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Luxen, André mailto [Université de Liège - ULg > Département de chimie (sciences) > Chimie organique de synthèse - Centre de recherches du cyclotron >]
Frère, Jean-Marie mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
2009
FEMS Microbiology Letters
Blackwell Publishing
300
42-47
Yes (verified by ORBi)
International
0378-1097
1574-6968
Oxford
United Kingdom
[en] Bacillus subtilis ; aldose-1-epimerase ; mutarotase ; GalM ; PBP4a ; DD-endopeptidase
[en] Abstract In Bacillus subtilis, the yoxA and dacC genes were proposed to form an operon. The yoxA gene was overexpressed in Escherichia coli and its product fused to a polyhistidine tag was purified. An aldose-1-epimerase or mutarotase activity was measured with the YoxA protein that we propose to rename as GalM by analogy with its counterpart in E. coli. The peptide d-Glu-delta-m-A(2)pm-d-Ala-m-A(2)pm-d-Ala mimicking the B. subtilis and E. coli interpeptide bridge was synthesized and incubated with the purified dacC product, the PBP4a. A clear dd-endopeptidase activity was obtained with this penicillin-binding protein, or PBP. The possible role of this class of PBP, present in almost all bacteria, is discussed.
Centre d'Ingénierie des Protéines - CIP ; Centre de Recherches du Cyclotron - CRC
PAI, ARC et EUR-INTAFAR
Researchers ; Professionals
http://hdl.handle.net/2268/24130
10.1111/j.1574-6968.2009.01761.x

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