Reference : Evidence for a precursor of the high-affinity metastasis-associated murine laminin recep...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Human health sciences : Oncology
Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor.
Rao, C. N. [> > > >]
Castronovo, Vincenzo mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases >]
Schmitt, M. C. [> > > >]
Wewer, U. M. [> > > >]
Claysmith, A. P. [> > > >]
Liotta, L. A. [> > > >]
Sobel, M. E. [> > > >]
American Chemical Society
Yes (verified by ORBi)
[en] Amino Acid Sequence ; Animals ; Base Sequence ; DNA/genetics ; Humans ; Immunoblotting ; Laminin/metabolism ; Mice ; Molecular Sequence Data ; Neoplasm Metastasis ; Nucleic Acid Conformation ; Nucleic Acid Hybridization ; Protein Biosynthesis ; Protein Conformation ; Protein Precursors/genetics/metabolism ; RNA, Messenger ; Rats ; Receptors, Immunologic/genetics/metabolism ; Receptors, Laminin ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; Tumor Cells, Cultured
[en] The high-affinity cellular receptor for the basement membrane component laminin is differentially expressed during tumor invasion and metastasis. A cDNA clone encoding the murine laminin receptor was isolated and identified on the basis of sequence homology to the human laminin receptor [Wewer et al. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 7137-7141]. Primer extension experiments demonstrated that the clone contained the complete 5' sequence of the murine laminin receptor mRNA. RNA blot data demonstrated a single-sized laminin receptor mRNA, approximately 1400 bases long, in human, mouse, and rat. The nascent laminin receptor predicted from the cDNA sequence is 295 amino acids long, with a molecular weight of 33,000, and contains one intradisulfide bridge, a short putative transmembrane domain, and an extracellular carboxy-terminal region which has abundant glutamic acid residues and multiple repeat sequences. The precursor of the laminin receptor is apparently smaller than the 67-kilodalton protein isolated from tissue. The apparent molecular weight on SDS-polyacrylamide gels of the rabbit reticulocyte cell-free translation product of selectively hybridized laminin receptor mRNA is 37,000. Antisera to three different domains of the cDNA-predicted receptor were used to study the relationship between the 37- and 67-kilodalton polypeptides. Antisera to cDNA-deduced synthetic peptides of the receptor immunoprecipitated a 37-kilodalton band both from cell-free translation products and from pulse-labeled cell extracts. On immunoblots of cell extracts, one antisynthetic peptide antiserum recognized only the 67-kilodalton receptor, while another antiserum identified both 37- and 67-kilodalton polypeptides, suggesting a precursor-product relationship between the two polypeptides.

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