Reference : Galectin-3, a Laminin Binding Protein, Fails to Modulate Adhesion of Human Melanoma Cell...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Human health sciences : Oncology
Galectin-3, a Laminin Binding Protein, Fails to Modulate Adhesion of Human Melanoma Cells to Laminin
van den Brule, F. A. [> > > >]
Buicu, C. [> > > >]
Sobel, M. E. [> > > >]
Liu, F. T. [> > > >]
Castronovo, Vincenzo mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire >]
Yes (verified by ORBi)
[en] laminin ; adhesion ; galectin-3 ; invasion ; metastasis ; melanoma
[en] Galectin-3 is a laminin binding protein which expression is altered in a variety of human carcinomas including colon, breast and endometrium. In these tumors, we consistently observed a down regulation of galectin-3 expression related to increased aggressiveness. Galectin-3 belongs to a family of galactose-binding lectins and binds laminin through its numerous poly-N-acetyllactosamine chains. To date, the exact role of galectin-3 in the complex interactions between cancer cells and laminin has not been clearly defined. Adhesion of melanoma cells to laminin is a critical event during tumor invasion and metastasis. In this study, we explore the possibility that galectin-3 could modulate attachment of two human melanoma cell lines to laminin. A2058 and A375 melanoma cell expressed galectin-3 on their surface as demonstrated by immunofluorescence, and attached to laminin in an in vitro assay. We demonstrate that neither recombinant galectin-3 nor an affinity purified antigalectin-3 antiserum altered adhesion of A2058 or A375 melanoma cells to laminin. Our data strongly suggest that galectin-3 is not a key element in adhesion of the melanoma cells to laminin. These results are not surprising in light of the observation that galectin-3 expression is down regulated in cancer and that increased adhesion to laminin is a constant feature of invasive cancer cells.
BIOMED BMH1-CT92-0520 ; Association Contre le Cancer ; Association Sportive Contre le Cancer

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VAN DEN BRULE FA NEOPLASMA 1995.PDFPublisher postprint3.99 MBRequest copy

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