Reference : Synthesis and Evaluation of 3-(Dihydroxyboryl)benzoic Acids as d,d-Carboxypeptidase R...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/24106
Synthesis and Evaluation of 3-(Dihydroxyboryl)benzoic Acids as d,d-Carboxypeptidase R39 Inhibitors.
English
Inglis, Steven R. [> > > >]
Zervosen, Astrid mailto [Université de Liège - ULg > > Centre de recherches du cyclotron >]
Woon, Esther C.Y. [> > > >]
Gerards, Thomas mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie végétale >]
Teller, Nathalie [> > > >]
Fischer S, Delphine [> >]
Luxen, André mailto [Université de Liège - ULg > Département de chimie (sciences) > Chimie organique de synthèse - Centre de recherches du cyclotron >]
Schofield, Christopher J. [> > > >]
4-Sep-2009
Journal of Medicinal Chemistry
American Chemical Society
Yes (verified by ORBi)
International
0022-2623
1520-4804
Washington
DC
[en] Penicillin-binding protein ; boronic acid ; peptidoglycan
[en] Penicillin binding proteins (PBPs) catalyze steps in the biosynthesis of bacterial cell walls and are the targets for the beta-lactam antibiotics. Non-beta-lactam based antibiotics that target PBPs are of interest because bacteria have evolved resistance to the beta-lactam antibiotics. Boronic acids have been developed as inhibitors of the mechanistically related serine beta-lactamases and serine proteases; however, they have not been explored extensively as PBP inhibitors. Here we report aromatic boronic acid inhibitors of the d,d-carboxypeptidase R39 from Actinomadura sp. strain. Analogues of an initially identified inhibitor [3-(dihydroxyboryl)benzoic acid 1, IC(50) 400 muM] were prepared via routes involving pinacol boronate esters, which were deprotected via a two-stage procedure involving intermediate trifluorborate salts that were hydrolyzed to provide the free boronic acids. 3-(Dihydroxyboryl)benzoic acid analogues containing an amide substituent in the meta, but not ortho position were up to 17-fold more potent inhibitors of the R39 PBP and displayed some activity against other PBPs. These compounds may be useful for the development of even more potent boronic acid based PBP inhibitors with a broad spectrum of antibacterial activity.
Centre de Recherches du Cyclotron - CRC ; Centre d'Ingénierie des Protéines - CIP
EUR-INTAFAR
Researchers
http://hdl.handle.net/2268/24106
10.1021/jm9009718

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