ORBi: Hlavcak P. - Cell surface immunophenotype and gelatinase activity of the human breast carcinoma cell line (MCF-7/6) with functionally defective E-cadherin.

Reference : Cell surface immunophenotype and gelatinase activity of the human breast carcinoma cell ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology Human health sciences : Oncology
	To cite this reference:	http://hdl.handle.net/2268/24089

Title :	Cell surface immunophenotype and gelatinase activity of the human breast carcinoma cell line (MCF-7/6) with functionally defective E-cadherin.
Language :	English
Author, co-author :	Hlavcak, P. [> > > >]
	Sedlakova, O. [> > > >]
	Sedlak, J. [> > > >]
	Hunakova, L. [> > > >]
	Duraj, J. [> > > >]
	Sulikova, M. [> > > >]
	Bizik, J. [> > > >]
	Castronovo, Vincenzo [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases >]
	Chorvath, B. [> > > >]
Publication date :	1999
Journal title :	Neoplasma
Publisher :	Slovak Academic Press Ltd.
Volume :	46
Issue/season :	1
Pages :	12-6
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0028-2685
City :	Bratislava
Country :	Slovakia
Keywords :	[en] Antigens, CD15/metabolism ; Antigens, CD44/metabolism ; Antigens, Surface/genetics ; Breast Neoplasms/enzymology/genetics/immunology ; Cadherins/metabolism ; Female ; Flow Cytometry ; Gelatinases/metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; Immunophenotyping ; Tumor Cells, Cultured ; Up-Regulation
Abstract :	[en] Expression of differentiation and adhesion cell surface antigens (LewisX - CD15, CD44, syndecan 1 - CD138 and basigin/EMMPRIN - CD147) were determined on the cell surface of human breast carcinoma MCF7 cells in vitro with the aid of flow cytometry and compared with that of MCF-7/6 cells, with functionally defective E-cadherin system and increased biological aggressiveness. The major cell surface alterations in MCF-7/6 cells compared with the parental MCF-7 cell line were a markedly increased CD15 (LewisX) and CD44 antigen cell surface expression on MCF-7/6 cells. There were no major differences between parental MCF-7 and MCF-7/6 cells in cell surface syndecan 1, basigin/EMMPRIN, E-cadherin and high affinity non-integrin laminin receptor expression. The constitutive cell surface gelatinase A and B activities were absent on MCF-7 and faint in MCF-7/6 cells. Both phorbol ester TPA and tumor necrosis factor TNF-alpha induced a marked up-regulation of gelatinase B only in MCF-7/6 cells. No marked differences in penetration of MCF-7 vs. MCF-7/6 cells into collagen/fibroblast matrix in vitro were observed. The increased expression of CD15 (LewisX), CD44 antigen and TNF-alpha-inducible gelatinase B on MCF-7/6 cells may represent auxiliary factors contributing to the increased biological aggressiveness of MCF-7/6 cells.
Permalink :	http://hdl.handle.net/2268/24089

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