Combined Interferon-Gamma and Tumor Necrosis Factor-Alpha Treatment Differentially Affects Adhesion and Migration of Keratinocyte-Derived Cells to Laminin-1
van den Brule, Frédéric A; Clausse, Nathalie; Delvenne, Philippeet al.
2000 • In Cell Adhesion and Communication, 7 (4), p. 321-9
[en] Interactions with the extracellular matrix constitute basic steps in cervix carcinoma cell invasion. In this study, we examined the adhesion and migration profiles of two human papillomavirus (HPV) DNA-transfected keratinocyte-derived cell lines, EIL8 and 18-11S3, and of the cervix adenocarcinoma SiHa cell line, towards laminin-1, and the selective effect of a 24-72 h treatment of 1000 U/ml interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha), a treatment that significantly decreases cervix carcinoma cell proliferation and progression in nude mice, on these parameters. Compared to normal cervix keratinocytes (CK) and two HPV DNA-transfected keratinocyte cell lines, in basal conditions, the SiHa cell line was characterized by increased attachment (SiHa, 48.74 +/- 4.02 vs. normal keratinocytes, 4.32 +/- 0.40, EIL8, 17.80 +/- 3.03 and 18-11S3, 17.82 +/- 1.48% of attached cells after 30 min) and marked directed chemotactic migration towards laminin-1. Interestingly, treatment of the cells with the cytokines (1000 U/ml IFN-gamma and TNF-alpha) did not modulate the adhesion properties of the cells, but chemotactic migration of SiHa cells to laminin-1 was significantly decreased, while migration towards type I collagen was increased. Similar results were obtained with the Ca Ski cervix carcinoma cell line. Our results emphasize the altered pattern of interactions of cervix carcinoma cells with extracellular matrix components such as laminin-1, compared to normal and pre-neoplastic cells, and contributes to the understanding of the effects of cytokine treatment on cervix carcinoma cells.
Delvenne, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Franzen-Detrooz, Elisabeth
Castronovo, Vincenzo ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire
Language :
English
Title :
Combined Interferon-Gamma and Tumor Necrosis Factor-Alpha Treatment Differentially Affects Adhesion and Migration of Keratinocyte-Derived Cells to Laminin-1
Publication date :
January 2000
Journal title :
Cell Adhesion and Communication
ISSN :
1061-5385
eISSN :
1026-7883
Publisher :
Taylor & Francis, United Kingdom
Volume :
7
Issue :
4
Pages :
321-9
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] European BIOMED contract BMH1-CT92-0520 Association Sportive contre le Cancer Fonds Léon Fredericq [BE] Télévie [BE]
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