Article (Scientific journals)
Histone deacetylases inhibitors as anti-angiogenic agents altering vascular endothelial growth factor signaling
Deroanne, Christophe; Bonjean, Karine; Servotte, Sandrine et al.
2002In Oncogene, 21 (3), p. 427-436
Peer Reviewed verified by ORBi
 

Files


Full Text
DEROANNE ONCOGENE 2002.pdf
Publisher postprint (682.95 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
angiogenesis; trichostatin A; suberoylanilide hydroxamic acid; VEGF; neuropilin-1; semaphorin III
Abstract :
[en] Angiogenesis is a complex biological process involving the coordinated modulation of many genes. Histone deacetylases (HDAC) are a growing family of enzymes that mediate the availability of chromatin to the transcriptional machinery. Trichostatin-A (TSA) and suberoylanilide hydroxamic acid (SAHA), two HDAC inhibitors known to relieve gene silencing, were evaluated as potential antiangiogenic agents. TSA and SAHA were shown to prevent vascular endothelial growth factor (VEGF)-stimulated human umbilical cord endothelial cells (HUVEC) from invading a type I collagen gel and forming capillary-like structures. SAHA and TSA inhibited the VEGF-induced formation of a CD31-positive capillary-like network in embryoid bodies and inhibited the VEGF-induced angiogenesis in the CAM assay. TSA also prevented, in a dose-response relationship, the sprouting of capillaries from rat aortic rings. TSA inhibited in a dose-dependent and reversible fashion the VEGF-induced expression of VEGF receptors, VEGFR1, VEGFR2, and neuropilin-1. TSA and SAHA upregulated the expression by HUVEC of semaphorin III, a recently described VEGF competitor, at both mRNA and protein levels. This effect was specific to endothelial cells and was not observed in human fibroblasts neither in vascular smooth muscle cells. These observations provide a conspicuous demonstration that HDAC inhibitors are potent anti-angiogenic factors altering VEGF signaling.
Disciplines :
Oncology
Biochemistry, biophysics & molecular biology
Author, co-author :
Deroanne, Christophe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal.
Bonjean, Karine 
Servotte, Sandrine
Devy, Laetitia
Colige, Alain ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal.
Clausse, Nathalie
Blacher, Silvia ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Verdin, Eric
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Nusgens, Betty ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Castronovo, Vincenzo ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire
Language :
English
Title :
Histone deacetylases inhibitors as anti-angiogenic agents altering vascular endothelial growth factor signaling
Publication date :
17 January 2002
Journal title :
Oncogene
ISSN :
0950-9232
eISSN :
1476-5594
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
21
Issue :
3
Pages :
427-436
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
CAC - Centre anticancéreux près l'Université de Liège asbl [BE]
Télévie [BE]
Available on ORBi :
since 28 September 2009

Statistics


Number of views
66 (12 by ULiège)
Number of downloads
2 (2 by ULiège)

Scopus citations®
 
367
Scopus citations®
without self-citations
354
OpenCitations
 
313

Bibliography


Similar publications



Contact ORBi