ORBi: Bucciarelli E. - Low dentin matrix protein 1 expression correlates with skeletal metastases development in breast cancer patients and enhances cell migratory capacity in vitro

Reference : Low dentin matrix protein 1 expression correlates with skeletal metastases development i...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology Human health sciences : Oncology
	To cite this reference:	http://hdl.handle.net/2268/24065

Title :	Low dentin matrix protein 1 expression correlates with skeletal metastases development in breast cancer patients and enhances cell migratory capacity in vitro
Language :	English
Author, co-author :	Bucciarelli, E. [> > > >]
	Sidoni, A. [> > > >]
	Bellezza, G. [> > > >]
	Cavaliere, A. [> > > >]
	Brachelente, G. [> > > >]
	Costa, G. [> > > >]
	Chaplet, M. [> > > >]
	Castronovo, Vincenzo [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire >]
	Bellahcene, Akeila [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases >]
Publication date :	Sep-2007
Journal title :	Breast Cancer Research and Treatment
Publisher :	Springer
Volume :	105
Issue/season :	1
Pages :	95-104
Audience :	International
ISSN :	0167-6806
City :	New York
Keywords :	[en] dentin matrix protein 1 ; breast cancer ; skeletal metastases ; migration ; breast cancer cell lines ; prognosis
Abstract :	[en] Small integrin-binding ligand N-linked glycoproteins (SIBLINGs) constitute a family of extracellular matrix proteins involved in bone homeostasis. Their pattern of expression has been primarily reported in bone and tooth and, more recently, in several cancer types. Dentin matrix protein 1 (DMP1), a SIBLING family member, expression was investigated by immunohistochemistry in a retrospective series of 148 primary human breast cancers. Correlations between DMP1 expression levels in the tumors and clinicopathologic features, bone metastases development and relapse of the disease were examined. DMP1 was expressed by 63.5% of the breast tumors analyzed. Significant inverse associations were found between DMP1 expression levels and the size and grade of the tumors (both, P < 0.0001). High DMP1 expression levels in the primary breast lesions were associated with a lower risk of subsequent development of skeletal metastases (P = 0.009). Patients with tumors expressing high levels of DMP1 had a significantly higher disease-free survival rate than those with low DMP1-expressing tumors (P = 0.0062). When DMP1 expression was examined in breast cancer cell lines, we found that non invasive MCF-7 and T47-D cells expressed higher levels than highly invasive MDA-MB-231 and Hs578T cells. Moreover, the specific inhibition of DMP1 expression in MCF-7 cells using siRNAs promoted significantly their migratory capability. Our data implicate for the first time DMP1 expression in breast cancer progression and bone metastases development.
Funders :	Fondazione Cassa di Risparmio di Perugia ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Inter-University Attraction Pole (IAP-P5/31) ; Université de Liège (Fonds Spéciaux) ; European-Commission (METABRE)
Permalink :	http://hdl.handle.net/2268/24065

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