Biotinylation; Bone Neoplasms/metabolism/secondary; Bone and Bones; Breast Neoplasms/metabolism/pathology; Cell Line, Tumor; Cell Membrane; Female; Gene Expression; Gene Expression Regulation, Neoplastic; Humans; Mass Spectrometry; Proteome/metabolism
Abstract :
[en] Metastatic breast cancer cells are characterized by their high propensity to colonize the skeleton and form bone metastases, causing major morbidity and mortality. Identifying key proteins involved in the osteotropic phenotype would represent a major step toward the development of both new prognostic markers and new effective therapies. Cell surface proteins differentially expressed in cancer cells are preferred potential targets for antibody-based targeted therapies. In this study, using cell surface biotinylation and a mass spectrometric approach, we have compared the profile of accessible cell surface proteins between the human breast cancer cell line MDA-MB-231 and its highly osteotropic B02 subclone. This strategy allowed the identification of several proteins either up- or downregulated in the osteotropic cell line, and differential protein expressions were validated using antibody-based techniques. Class I HLAs were down-regulated in the bone metastatic variant, whereas alpha(v)beta(3) integrins, among others, were consistently up-regulated in this latter cell line. These results show that comprehensive profiling of the cell surface proteome of mother cancerous cell lines and derived organ-specific metastatic cell lines provides an effective approach for the identification of potential accessible marker proteins for both prognosis and antibody-based targeted therapies.
Kischel, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases
Guillonneau, Francois
Dumont, Bruno ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases
Bellahcene, Akeila ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases
Stresing, Verena
Clezardin, Philippe
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Castronovo, Vincenzo ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases
Language :
English
Title :
Cell membrane proteomic analysis identifies proteins differentially expressed in osteotropic human breast cancer cells.
Publication date :
2008
Journal title :
Neoplasia
ISSN :
1522-8002
eISSN :
1476-5586
Publisher :
BC Decker, Hamilton Ont, Canada
Volume :
10
Issue :
9
Pages :
1014-20
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
EU - European Commission F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] Télévie [BE] CAC - Centre anticancéreux près l'Université de Liège asbl [BE] Fonds Léon Fredericq [BE] Tournesol
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