Reference : Cell membrane proteomic analysis identifies proteins differentially expressed in oste...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Human health sciences : Oncology
http://hdl.handle.net/2268/24060
Cell membrane proteomic analysis identifies proteins differentially expressed in osteotropic human breast cancer cells.
English
Kischel, Philippe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases >]
Guillonneau, Francois [> > > >]
Dumont, Bruno mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases >]
Bellahcene, Akeila mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases >]
Stresing, Verena [> > > >]
Clezardin, Philippe [> > > >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Castronovo, Vincenzo mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases >]
2008
Neoplasia : An International Journal for Oncology Research
BC Decker
10
9
1014-20
Yes (verified by ORBi)
International
1522-8002
1476-5586
Hamilton Ont
Canada
[en] Biotinylation ; Bone Neoplasms/metabolism/secondary ; Bone and Bones ; Breast Neoplasms/metabolism/pathology ; Cell Line, Tumor ; Cell Membrane ; Female ; Gene Expression ; Gene Expression Regulation, Neoplastic ; Humans ; Mass Spectrometry ; Proteome/metabolism
[en] Metastatic breast cancer cells are characterized by their high propensity to colonize the skeleton and form bone metastases, causing major morbidity and mortality. Identifying key proteins involved in the osteotropic phenotype would represent a major step toward the development of both new prognostic markers and new effective therapies. Cell surface proteins differentially expressed in cancer cells are preferred potential targets for antibody-based targeted therapies. In this study, using cell surface biotinylation and a mass spectrometric approach, we have compared the profile of accessible cell surface proteins between the human breast cancer cell line MDA-MB-231 and its highly osteotropic B02 subclone. This strategy allowed the identification of several proteins either up- or downregulated in the osteotropic cell line, and differential protein expressions were validated using antibody-based techniques. Class I HLAs were down-regulated in the bone metastatic variant, whereas alpha(v)beta(3) integrins, among others, were consistently up-regulated in this latter cell line. These results show that comprehensive profiling of the cell surface proteome of mother cancerous cell lines and derived organ-specific metastatic cell lines provides an effective approach for the identification of potential accessible marker proteins for both prognosis and antibody-based targeted therapies.
Giga-Cancer
European Commison FP6 (STROMA - METABRE) ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; TELEVIE ; Centre Anticancéreux près l'Université de Liège ; Fonds Léon Fredericq ; Tournesol
http://hdl.handle.net/2268/24060

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
neo08570.pdfPublisher postprint1.14 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.