Reference : Selective osteopontin knockdown exerts anti-tumoral activity in a human glioblastoma mod...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Human health sciences : Oncology
http://hdl.handle.net/2268/24054
Selective osteopontin knockdown exerts anti-tumoral activity in a human glioblastoma model.
English
Lamour, Virginie mailto [Université de Liège - ULg > > Centre facultaire de rech. en cancérologie expérimentale >]
Le Mercier, M. [> > > >]
Lefranc, F. [> > > >]
Hagedorn, M. [> > > >]
Javerzat, S. [> > > >]
Bikfalvi, A. [> > > >]
Kiss, R. [> > > >]
Castronovo, Vincenzo mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases >]
Bellahcene, Akeila mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases >]
2010
International Journal of Cancer = Journal International du Cancer
Wiley Liss, Inc.
126
1797-1805
Yes (verified by ORBi)
International
0020-7136
1097-0215
New York
NY
[en] osteopontin ; SIBLINGs ; glioma ; migration ; proliferation
[en] Osteopontin (OPN), a member of the SIBLING (Small Integrin-Binding LIgand N-linked Glycoprotein) family, is overexpressed in human glioblastoma. Higher levels of OPN expression correlate with increased tumor grade and enhanced migratory capacity of tumor cells. Based on these observations, we explored the possibility that knocking down OPN expression in glioblastoma cells could exert an anti-tumoral activity using an avian in vivo glioblastoma model that mimics closely human gliobastoma. Human U87-MG glioma cells transfected with specific anti-OPN small interfering RNAs (siRNAs) were grafted onto the chicken chorio-allantoic membrane (CAM). OPN-deficient U87-MG cells gave rise to tumors that were significantly smaller than tumors formed from untransfected cells (paired t-test, p<0.05). Accordingly, the amount of proliferating cells in OPN-deficient tumors showed a six-fold reduction when compared to control tumors. However, OPN inhibition did not affect significantly tumor-associated angiogenesis. In vitro, OPN-silenced U87-MG and U373-MG cells showed decreased motility and migration. This is the first demonstration that OPN inhibition blocks glioma tumor growth, making this invasion-related protein an attractive target for glioma therapy. (c) 2009 UICC.
Giga-Cancer
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
http://hdl.handle.net/2268/24054
10.1002/ijc.24751

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