|Reference : A new natural hGH variant--17.5 kd--produced by alternative splicing. An additional cons...|
|Scientific journals : Article|
|Life sciences : Biochemistry, biophysics & molecular biology|
|A new natural hGH variant--17.5 kd--produced by alternative splicing. An additional consensus sequence which might play a role in branchpoint selection|
|Lecomte, C. M. [ > > ]|
|Renard, A. [ > > ]|
|Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]|
|Nucleic Acids Research|
|Oxford University Press|
|Yes (verified by ORBi)|
|[en] Base Sequence ; Chorionic Gonadotropin/*genetics ; DNA/genetics ; Genes ; Genes, Regulator ; Humans ; Molecular Weight ; Pituitary Neoplasms/analysis ; *RNA Splicing ; RNA, Neoplasm/genetics/isolation & purification ; Recombinant Proteins/genetics|
|[en] From a human pituitary cDNA library, we have cloned 3 distinct human growth hormone (hGH) cDNAs, coding respectively for the 22 K hGH, the 20 K variant, and a yet unknown 17.5 K variant. S1 mapping analysis using human pituitary RNA confirms the existence of at least four distinct hGH mRNAs originating from alternative acceptor sites at the second intron of the primary transcript. We have analysed the hGH gene sequence to explain the high frequency of alternative splicings which occur only at this location. In this study we propose CTTGNNPyPyPy as an additional consensus sequence guiding the selection of the branched nucleotide.|
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