Reference : Molecular cloning and characterization of two forms of trout growth hormone cDNA: exp...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/23690
Molecular cloning and characterization of two forms of trout growth hormone cDNA: expression and secretion of tGH-II by Escherichia coli
English
Rentier-Delrue, Françoise mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique >]
Swennen, D. [> > > >]
Mercier, L. [> > > >]
Lion, M. [ > > ]
Benrubi, O. [> > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
1989
DNA
Mary Ann Liebert, Inc.
8
2
109-17
Yes (verified by ORBi)
0198-0238
New York
NY
[en] Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; *Dna ; Escherichia coli/metabolism ; Growth Hormone/*genetics/secretion ; Molecular Sequence Data ; Salmon ; Salmonidae/*genetics ; Sequence Homology, Nucleic Acid ; Trout/*genetics
[en] We constructed a cDNA library using mRNA isolated from rainbow trout pituitaries. Two types of cDNA clones encoding growth hormone (GH) were isolated and their complete nucleotide sequences determined. Twenty seven nucleotide substitutions in the coding region and 108 in the noncoding region distinguish the cDNAs of trout GH-I and II. Both cDNAs encode polypeptides of 210 amino acids, including a putative signal peptide of 22 amino acids, which differ by 12 residues. In both trout and salmon, GH-I mRNA is predominant, which suggests that the variation in the amount of secreted GH originates from a transcriptional event. Moreover, comparison of rainbow trout and chum salmon GH reveals that, in both cases, the predominant GH-I has mutated less than its GH-II counterpart. Mature tGH-II was expressed in Escherichia coli using the pIN-III-ompA-Hind secretion vector.
http://hdl.handle.net/2268/23690
1989/03/01

There is no file associated with this reference.

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.