No full text
Article (Scientific journals)
Isolation of follicular dendritic cells from human tonsils and adenoids. V. Effect on lymphocyte proliferation and differentiation.
Cormann, N.; Lesage, F.; Heinen, Ernst et al.
1986In Immunology Letters, 14 (1), p. 29-35
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Adenoids/cytology; B-Lymphocytes/immunology; Cell Differentiation; Cell Separation; Cells, Cultured; Child; Child, Preschool; Dendritic Cells/cytology/immunology; Humans; Immunoglobulin G/secretion; Lymphocyte Activation; Palatine Tonsil/cytology; Phytohemagglutinins/pharmacology; Pokeweed Mitogens/pharmacology; T-Lymphocytes/immunology
Abstract :
[en] Follicular dendritic cells (FDC) are located only in lymph follicles and are characterized by their capacity to retain high amounts of immune complexes on their plasma membranes. As their functions in germinal centres are unknown, we isolated them from human tonsils and cultured them with autologous lymphoid cells. Cultures of lymphoid cells alone or with added macrophages were used as controls. Lymphoid cells incorporated tritiated thymidine only when FDC and lectins were added; this could be shown after several periods of time. However, the Ig secretion by lymphoid cell populations was inhibited by FDC after several days in vitro. In contrast, the supernatants of lymphocytes cultured alone or with macrophages only for the same periods of time contained increasing amounts of immunoglobulins. This inhibitory effect of FDC on immunoglobulin production was observed for all considered isotypes. Our data suggest that FDC stimulate lymphoid cell proliferation but reduce B-cell differentiation. This is the first accessory cell activity definitely shown for FDC in cultures.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Cormann, N.
Lesage, F.
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Schaaf-Lafontaine, Nicole ;  Université de Liège - ULiège > Hématologie biologique et immuno hématologie
Kinet-Denoel, C.
Simar, L. J.
Language :
English
Title :
Isolation of follicular dendritic cells from human tonsils and adenoids. V. Effect on lymphocyte proliferation and differentiation.
Publication date :
1986
Journal title :
Immunology Letters
ISSN :
0165-2478
eISSN :
1879-0542
Publisher :
Elsevier Science
Volume :
14
Issue :
1
Pages :
29-35
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 September 2009

Statistics


Number of views
56 (1 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
31
Scopus citations®
without self-citations
22
OpenCitations
 
35

Bibliography


Similar publications



Contact ORBi