Reference : Inhibitory effect of curcuminoids and tetrahydrocurcuminoids on equine activated neutrop...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
Life sciences : Veterinary medicine & animal health
Life sciences : Biotechnology
http://hdl.handle.net/2268/5488
Inhibitory effect of curcuminoids and tetrahydrocurcuminoids on equine activated neutrophils and myeloperoxidase activity
English
Franck, Thierry mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Kohnen, Stephan mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Grulke, Sigrid mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés >]
Neven, Philippe mailto [> > > >]
Goutman, Yvette [> > > >]
Péters, Fabrice [> >]
Pirotte, Bernard mailto [Université de Liège - ULg > > Chimie pharmaceutique >]
Deby, Ginette [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
2008
Physiological Research
57
4
577-587
Yes (verified by ORBi)
International
0862-8408
1802-9973
Prague
Czech Republic
[en] curcuminoids ; stimulated equine neutrophils ; myeloperoxidase activity
[en] In the horse, the inflammation response to various pathologies (intestinal strangulations, laminitis, etc.) involves an excessive stimulation of the polymorphonuclear neutrophils releasing reactive oxygen species (ROS) and myeloperoxidase (MPO). The aim of the present work was to study the effect of natural polyphenols, curcuminoids and tetrahydrocurcuminoids (THC) on isolated stimulated equine neutrophils and on the activity of purified MPO. The ROS production and the release of MPO by activated neutrophils were measured by chemiluminescence and ELISA techniques, respectively. The activity of purified MPO was measured by studying its nitration, chlorination or oxidation capacity and by using an original method called SIEFED allowing the study of drug interaction with the enzyme without interferences of the medium. Curcuminoids and THC had dose-dependent inhibitory effects on ROS production and MPO release by activated neutrophils and on purified MPO activity. We suggest that the higher efficacy of curcuminoids versus THC could be explained, at least partially, by its chemical structure: the conjugated double bounds and the plane structure of curcuminoids made easier the neutralization of the radical species generated by activated neutrophils and the interaction of the drug with the active site of MPO. These inhibitory effects of curcuminoids on the oxidant activity of equine neutrophils and on MPO activity open therapeutic perspectives in equine pathologies with excessive inflammatory reactions.
Centre de l’Oxygène, Recherche et Développement - CORD
Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
MPOEQUI
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/5488
also: http://hdl.handle.net/2268/23531

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