Article (Scientific journals)
Temperature dependence of mycosubtilin homologue production in Bacillus subtilis ATCC6633.
Fickers, Patrick; Leclere, Valerie; Guez, Jean*-Sebastien et al.
2008In Research in Microbiology, 159 (6), p. 449-57
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Keywords :
Bacillus subtilis/chemistry/genetics/growth & development/metabolism; Bacterial Proteins/genetics/metabolism; Gene Expression Regulation, Bacterial; Lipoproteins/chemistry/genetics/isolation & purification/metabolism; Multienzyme Complexes/genetics/metabolism; Temperature
Abstract :
[en] Bacillus subtilis ATCC6633 produces mycosubtilin, a non-ribosomally synthesized lipopeptide of the iturin family which presents antagonistic activities toward various phytopathogens. Different homologues with fatty acid moiety varying from C15 to C17 are usually co-produced, with their biological activities increasing with the number of carbons in the fatty acid chain. In the present report, we highlight that growth temperature modulates both the extent of mycosubtilin production and the relative abundance of the different homologues. A 30-fold increase in mycosubtilin production was observed when the temperature was decreased from 37 degrees C to 25 degrees C for both strain ATCC6633 and its derivative BBG100, a constitutive mycosubtilin overproducer. However, no significant difference in either the expression of the mycosubtilin synthetase encoding genes or in the intracellular synthetase concentration could be found, suggesting that the observed phenotype originated from a higher mycosubtilin synthetase turnover at lower temperature. We also point out that lower growth temperature leads to an increased proportion of odd-numbered fatty acid homologues as a consequence of de novo synthesis of C17 anteiso fatty acid following cell adaptation to low temperatures.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Fickers, Patrick ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Leclere, Valerie
Guez, Jean*-Sebastien
Bechet, Max
Coucheney, Francoise
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines
Jacques, Philippe ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Language :
English
Title :
Temperature dependence of mycosubtilin homologue production in Bacillus subtilis ATCC6633.
Publication date :
2008
Journal title :
Research in Microbiology
ISSN :
0923-2508
eISSN :
1769-7123
Publisher :
Elsevier, Paris, France
Volume :
159
Issue :
6
Pages :
449-57
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 September 2009

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