ORBi: Fickers Patrick - High-level biosynthesis of the anteiso-C(17) isoform of the antibiotic mycosubtilin in Bacillus subtilis and characterization of its candidacidal activity.

Reference : High-level biosynthesis of the anteiso-C(17) isoform of the antibiotic mycosubtilin in B...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/23494

Title :	High-level biosynthesis of the anteiso-C(17) isoform of the antibiotic mycosubtilin in Bacillus subtilis and characterization of its candidacidal activity.
Language :	English
Author, co-author :	Fickers, Patrick [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
	Guez, Jean-Sebastien [> > > >]
	Damblon, Christian [Université de Liège - ULg > Département de chimie (sciences) > Chimie biologique structurale >]
	Leclere, Valerie [> > > >]
	Bechet, Max [> > > >]
	Jacques, Philippe [> > > >]
	Joris, Bernard [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines >]
Publication date :	2009
Journal title :	Applied & Environmental Microbiology
Publisher :	American Society for Microbiology (ASM)
Volume :	75
Issue/season :	13
Pages :	4636-40
Audience :	International
ISSN :	0099-2240
e-ISSN :	1098-5336
City :	Washington
Country :	DC
Keywords :	[en] Antibiotics, Antifungal/biosynthesis ; Bacillus subtilis/genetics/metabolism ; Biosynthetic Pathways/genetics ; Candida/drug effects ; Genes, Bacterial ; Genetic Engineering ; Lipoproteins/biosynthesis ; Microbial Sensitivity Tests ; Protein Isoforms/metabolism
Abstract :	[en] High-level production (880 mg liter(-1)) and isolation of the anteiso-C(17) isoform of the lipopeptide mycosubtilin produced by a genetically engineered Bacillus subtilis strain are reported. Antifungal activity of this isoform, as determined via culture and fluorometric and cell leakage assays, suggests its potential therapeutic use as an antifungal agent, in particular against Candida spp.
Permalink :	http://hdl.handle.net/2268/23494
DOI :	10.1128/AEM.00548-09

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