Reference : Overexpression of the platelet P2X1 ion channel in transgenic mice generates a novel pro...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/23397
Overexpression of the platelet P2X1 ion channel in transgenic mice generates a novel prothrombotic phenotype.
English
Oury, Cécile mailto [Katholieke Universiteit Leuven - KUL > Molecular and Cellular Medicine > CMVB > >]
Kuijpers, Marijke [Universiteit Maastricht > Biochemistry > CARIM > >]
Toth-Zsamboki, Emese [Katholieke Universiteit Leuven - KUL > Molecular and Cellular Medicine > CMVB > >]
Bonnefoy, Arnaud [Katholieke Universiteit Leuven - KUL > Molecular and Cellular Medicine > CMVB > >]
Danloy, Sophie [Katholieke Universiteit Leuven - KUL > Molecular and Cellular Medicine > CMVB > >]
Vreys, I. [Katholieke Universiteit Leuven - KUL > Molecular and Cellular Medicine > CMVB > >]
Feijge, Marion [Universiteit Maastricht > Biochemistry > CARIM > >]
De Vos, Rita [Katholieke Universiteit Leuven - KUL > Molecular and Cellular Medicine > Morphology and Molecular Pathology > >]
Vermylen, Jos [Katholieke Universiteit Leuven - KUL > Molecular and Cellular Medicine > CMVB > >]
Hoylaerts, Marc F [Katholieke Universiteit Leuven - KUL > Molecular and Cellular Medicine > CMVB > >]
2003
Blood
American Society of Hematology
Yes (verified by ORBi)
International
0006-4971
1528-0020
Washington
DC
[en] thrombosis ; purinergic receptors ; transgenic mice
[en] This study describes transgenic mice overexpressing the human P2X(1) ion channel in the megakaryocytic cell lineage. Platelets from these mice display increased secretion and aggregation evoked by low doses of collagen, convulxin, or the thromboxane A(2) mimetic U46619. Perfusing whole blood from transgenic mice over collagen fibers at a shear rate of 1000 seconds(-1) resulted in increased P2X(1)-dependent aggregate formation and phosphatidylserine exposure. Platelet hyperreactivity to collagen was correlated with up-regulated extracellular signal-regulated kinase 2 (ERK2) phosphorylation. In a viscometer, shear stress caused potent aggregation of transgenic platelets under conditions in which wild-type platelets did not aggregate. In an in vivo model of thromboembolism consisting of intravenous injection of a low dose of collagen plus epinephrine, transgenic mice died more readily than wild-type mice. Preinjection of U0126 not only fully protected transgenic mice against thrombosis, it also enhanced the survival of wild-type mice injected with a higher collagen dose. Hence, the platelet P2X(1) ion channel plays a role in hemostasis and thrombosis through its participation in collagen-, thromboxane A(2)-, and shear stress-triggered platelet responses. Activation of the ERK2 pathway is instrumental in these processes.
http://hdl.handle.net/2268/23397

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