[en] Monoclonal antibodies to oxytocin (OT) and vasopressin (VP) revealed some positively staining stromal cells in the subcapsular cortex and in the medulla of the human thymus. We further demonstrated that these cells are a subset of epithelial endocrine cells and also contain immunoreactive interleukin-1 together with the neuropeptides. In addition, the thymic cells stained by monoclonal antibodies directed to the cyclic part of oxytocin or vasopressin also contained some immunoreactive neurophysins. These data support the concept of intrathymic synthesis of neurohypophyseal-like peptides fitting the hypothalamic model. However, we observed that, contrary to the situation in the brain, OT- and VP-like peptides colocalized in the same thymic cells. Furthermore, one monoclonal antibody, specific for the tail part of oxytocin, did not label thymic cells. Therefore, thymic neuropeptide(s) could be related to, but distinct from, authentic OT and VP. These observations suggest some molecular differences between hypothalamic and thymic oxytocin biosynthetic pathways which need to be further investigated.
Disciplines :
Immunology & infectious disease
Author, co-author :
Robert, Françoise
Geenen, Vincent ; Université de Liège - ULiège > Centre d'immunologie - Embryologie
Schoenen, Jean ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Neuro-anatomie
Burgeon, Etienne
De Groote, Donat
Defresne, Marie-Paule ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Franchimont, Paul
Language :
English
Title :
Colocalization of immunoreactive oxytocin, vasopressin and interleukin-1 in human thymic epithelial neuroendocrine cells
Publication date :
1991
Journal title :
Brain, Behavior and Immunity
ISSN :
0889-1591
eISSN :
1090-2139
Publisher :
Academic Press, Orlando, United States - Florida
Volume :
5
Pages :
102-115
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
European Scientific Foundation Fund for Medical Scientific Research (Belgium) ULiège FSR - Université de Liège. Fonds spécial pour la recherche [BE]
Altstein, Whitnall, House, Key, Gainer (1988) An immunochemical analysis of oxytocin and vasopressin prohormone processing in vivo. Peptides 9:87-105.
Amico (1988) Oxytocin and oxytocin-like peptides in primate tissues and body fluids. Recent progress in posterior pituitary hormones , S. Yoshida, L. Share, Elsevier, New York; 207-213.
Baldino, O'Kane, Fitzpatrick-McElligott, Wolfson (1988) Coordinate hormonal and synaptic regulation of vasopressin messenger RNA. Science 241:978-981.
Breder, Dinarello, Saper (1988) Interleukin-1 immunoreactive innervation of the human hypothalamus. Science 240:321-324.
Burbach (1986) Proteolytic conversion of oxytocin, vasopressin and related peptides in the brain. Current topics in neuroendocrinology , D. Ganten, D. Pfaff, Springer-Verlag, Berlin; 15:55-90.
Burgeon, Chapleur, Schoenen, Remichius, Legros, Geenen, Robert (1991) Monoclonal antibodies to oxytocin Production and characterization. J Neuroimmunol, in press; .
Clements, Funder (1986) Arginine vasopressin (AVP) and AVP-like immunoreactivity in peripheral tissues. Endocr. Rev 7:449-460.
Dinarello (1989) Interleukin 1 and its biologically related cytokines. Adv. Immunol 44:153-205.
Eisenbarth, Walsh, Nirenberg (1979) Monoclonal antibody to a plasma membrane antigen of neurons. Proc. Natl. Acad. Sci. USA 76:4913-4917.
Elands, Resink, De Kloet (1988) Oxytocin receptors in the rat thymic gland. Eur. J. Pharmacol 151:345.
Elands, Resink, De Kloet (1990) Neurohypophyseal hormone receptors in the rat thymus, spleen, and lymphocytes. Endocrinology 126:2703-2710.
Ervin, Leake, Ross, Calvario, Fischer (1985) Arginine vasotocin in ovine fetal blood, urine, and amniotic fluid. J. Clin. Invest 75:1696-1701.
Ervin, Amico, Leake, Ross, Robinson, Fischer (1988) Arginine vasotocin and a novel oxytocin-vasotocin-like material in plasma of human newborns. Biol. Neonate 53:17-22.
Ervin, Polk, Humme, Ross, Leake, Fischer (1988) Immunoreactive vasotocin, vasopressin and oxytocin in ovine fetal and neonatal thymus glands. Clin. Res 36:217A.
Geenen, Legros, Franchimont, Baudrihaye, Defresne, Boniver (1986) The neuroendocrine thymus: Coexistence of oxytocin and neurophysin in the human thymus. Science 232:508-511.
Geenen, Legros, Franchimont, Defresne, Boniver, Ivell, Richter (1987) The thymus as a neuroendocrine organ Synthesis of vasopressin and oxytocin in human thymic epithelium. Annals of the New York Academy of Sciences 496:56-66.
Geenen, Defresne, Robert, Legros, Franchimont, Boniver (1988) The neurohormonal thymic microenvironment: Immunocytochemical evidence that thymic nurse cells are neuroendocrine cells. Neuroendocrinology 47:365-368.
Geenen, Robert, Fatemi, Defresne, Boniver, Legros, Franchimont (1988) Vasopressin and oxytocin: thymic signals and receptors in T cell ontogeny. Recent progress in posterior pituitary hormones , S. Yoshida, L. Share, Elsevier, New York; 303-310.
Gitelman, Klaper, Alderman, Blythe (1980) Ala-Gly- and Val-Asp-[Arg 8]-vasopressin: Bovine storage forms of arginine vasopressin with natriuretic activity. Science 207:893-896.
Haftek, Staquet, Viac, Schmitt, Thivolet (1984) Immunoelectron microscopic analysis of human keratinocyte differentiation-related antigens defined by monoclonal antibodies (McAbs). Special reference to desmosomes and keratin filaments , J. Invest. Dermatol; 82:541.
Hawthorn, Nussey, Henderson, Jenkins (1987) Immunohistological localization of oxytocin and vasopressin in the adrenal glands of rat, cow, hamster and guinea pig. Cell Tissue Res 250:1-6.
Haynes, Shimizu, Eisenbarth (1983) Identification of human and rodent thymic epithelium using tetanus toxin and monoclonal antibody A2B5. J. Clin. Invest 71:9-14.
Ivell, Richter (1984) The gene for the hypothalamic peptide hormone is highly expressed in the bovine corpus luteum: Biosynthesis, structure and sequence analysis. EMBO J 3:2351-2354.
Jordan, Robinson (1981) T lymphocyte differentiation. The thymus gland , M.D. Kendall, Academic Press, New York; 151-178.
Kai-Kai, Anderton, Keen (1986) A quantitative analysis of the interrelationships between subpopulations of rat sensory neurons containing arginine vasopressin or oxytocin and those containing substance P, fluoride-resistant acid phosphatase or neurofilament protein. Neuroscience 18:475-486.
Kendall (1981) The cells of the thymus. The thymus gland , M.D. Kendall, Academic Press, New York; 63-84.
Kiyama, Emson (1990) Evidence for the co-expression of oxytocin and vasopressin messenger ribonucleic acids in magnocellular neurosecretory cells: Simultaneous demonstration of two neurophysin messenger ribonucleic acids by hybridization histochemistry. J. Neuroendocrinol 2:257-259.
Land, Schuetz, Schmale, Richter (1982) Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin-neurophysin II precursor. Nature (London) 295:299-303.
Land, Grez, Ruppert, Schmale, Rehbein, Richter, Schuetz (1983) Deduced amino acid sequence from the bovine oxytocin-neurophysin I precursor cDNA. Nature (London) 302:342-344.
Laster, Itoh, Palker, Haynes (1986) The human thymic microenvironment: Thymic epithelium contains specific keratins associated with early and late stages of epidermal keratinocyte maturation. Differentiation 31:67-77.
Le, Tuck, Dinarello, Haynes, Singer (1987) Human thymic epithelial cells produce interleukin 1. J. Immunol 138:2520-2526.
Lechan, Toni, Clark, Cannon, Shaw, Dinarello, Reichlin (1990) Immunoreactive interleukin 1β localization in the rat forebrain. Brain Res 514:135-140.
Lefebvre, Paradis, Zingg (1990) Extrahypothalamic gene expression. Third International Vasopressin Conference , Montpellier, France; A2.
Legros (1975) The radioimmunoassay of human neurophysins: Contribution to the understanding of the physiopathology of neurohypophyseal function. Ann. N.Y. Acad. Sci 248:281-303.
Liu, Poulter, Neacsu, Burbach (1988) Isolation and identification of vasopressin- and oxytocin-immunoreactive substances from bovine pineal gland. Presence of Nα-acetyloxytocin , J. Biol. Chem; 263:72-75.
Moll, Lane, Robert, Geenen, Legros (1988) The neuroendocrine thymus. Abundant occurrence of oxytocin-, vasopressin-, and neurophysin-like peptides in epithelial cells , Histochemistry; 89:385-390.
Murphy, Hwee Luan, Ngee Chih, Carter (1990) Gonadal expression of neuropeptide genes in normal and transgenic animals. Third International Vasopressin Conference , Montpellier, France; A3.
Nicolas, Reano, Kaiserlian, Thivolet (1986) Epithelial cell heterogeneity in the guinea pig thymus: Immunohistochemical characterization of four thymic epithelial subsets defined by monoclonal anti-keratin antibodies. Eur. J. Immunol 16:457-464.
Nicolas, Reano, Kaiserlian, Thivolet (1989) Epithelial cell heterogeneity in mammalian thymus: Monoclonal antibody to high molecular weight keratins exclusively binds to Hassall's corpuscles. Histochem. J 21:357-364.
Nieuwenhuis (1984) Arginine vasotocin (AVT), an alleged hormone of the mammalian pineal gland. Life Sci 35:1713-1724.
Reuter, Bernier, Gysen, Gevaert, Gathy, Lopez, Dupont, Damas, Franchimont (1988) A RIA for tumor necrosis factor (TNF α) and interleukin 1 β (IL-1β) and the direct determination in serum. Prog. Leukocyte Biol 8:377-381.
Robert, Léon-Henri, Chapleur-Chateau, Girr, Burlet (1985) Comparison of three immunoassays in the screening and characterization of monoclonal antibodies against arginine-vasopressin. J. Neuroimmunol 9:205-220.
Rosenbaum, Zimmerman, Nilaver (1990) Identification of pro-pressophysin-related immunoreactivity in bovine pituitary gland. Neuroendocrinology 52(1 S):110.
Savino, Dardenne (1988) Immunohistochemical studies on a human thymic epithelial cell subset defined by the anti-cytokeratin 18 monoclonal antibody. Cell Tissue Res 254:225-231.
Smith, McIntosh (1983) Neurohypophyseal peptides in the human fetus: Presence in pituitary extracts of immunoreactive arginine vasotocin. J. Endocrinol 99:441-445.
Stefanini, De Martino, Zamboni (1967) Fixation of ejaculated spermatozoa for electron microscopy. Nature (London) 216:173-174.
Swann, O'Shaughnessy, Birkett, Wathes, Porter, Pickering (1984) Biosynthesis of oxytocin in the corpus luteum. FEBS Lett 174:262-266.
Vandesande, Dierickx (1975) Identification of the vasopressin producing and the oxytocin producing neurons in the hypothalamic magnocellular neurosecretory system of the rat. Cell Tissue Res 164:153-162.
Viac, Reano, Brochier, Staquet, Thivolet (1983) Reactivity pattern of a monoclonal antikeratin antibody (KL-1). J. Invest. Dermatol 81:351-354.
