ORBi: Tsunoda R. - Human follicular dendritic cells in vitro and follicular dendritic-cell-like cells.

Reference : Human follicular dendritic cells in vitro and follicular dendritic-cell-like cells.


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Anatomy (cytology, histology, embryology...) & physiology
	To cite this reference:	http://hdl.handle.net/2268/23314

Title :	Human follicular dendritic cells in vitro and follicular dendritic-cell-like cells.
Language :	English
Author, co-author :	Tsunoda, R. [> > > >]
	Bosseloir, A. [> > > >]
	Onozaki, K. [> > > >]
	Heinen, Ernst [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Miyake, K. [> > > >]
	Okamura, H. [> > > >]
	Suzuki, K. [> > > >]
	Fujita, T. [> > > >]
	Simar, L. J. [> > > >]
	Sugai, N. [> > > >]
Publication date :	1997
Journal title :	Cell & Tissue Research
Publisher :	Springer Science & Business Media B.V.
Volume :	288
Issue/season :	2
Pages :	381-9
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0302-766X
e-ISSN :	1432-0878
City :	New York
Country :	NY
Keywords :	[en] Cell Adhesion/physiology ; Cell Division/drug effects ; Cells, Cultured ; Cytokines/pharmacology ; Dendritic Cells/physiology ; Germinal Center/cytology/drug effects ; Humans ; Immunophenotyping ; Interleukin-6/biosynthesis ; Lymphocytes/cytology/drug effects ; Recombinant Proteins/pharmacology
Abstract :	[en] Human follicular dendritic cell (FDC)-like cells (FLC) have been utilized for the in vitro analysis of germinal center reactions. However, there is no consensus whether FLC represent FDC in vitro. The purpose of the present study has therefore been to determine distinguishing features of FDC and FLC in vitro. The expression of CD40, CD54, CD49d, cytokine (gamma-IFN and IL-4)-dependent MHC-class II, and CD106 was observed to be specific for the determination of FDC in long-term culture. The cytokine-dependent emperipolesis of germinal center B cells was establised as another discriminating property for FDC in vitro. In 2 out of 72 long-term cultures of FDC, we encountered dividing cells among the non-dividing population of FDC. The dividing cells expressed accessory molecules similar to those of FDC but showed emperipolesis only for the initial few days of their growth. FDC did not enhance the CD40-dependent proliferation of germinal center B cells; in contrast, FLC augumented it. Both types of cells produced a significant amount of cytokine-dependent IL-6. Further studies are needed to determine whether FLC originate from FDC in vitro.
Permalink :	http://hdl.handle.net/2268/23314

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