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| Reference : The influence of follicular dendritic cells on B-cell proliferation depends on the activ... |
| Scientific journals : Article | |||
| Life sciences : Anatomy (cytology, histology, embryology...) & physiology | |||
| http://hdl.handle.net/2268/23312 | |||
| The influence of follicular dendritic cells on B-cell proliferation depends on the activation of B cells and the mitogen used. | |
| English | |
| Bosseloir, A. [> > > >] | |
| Bouzahzah, F. [> > > >] | |
| Defrance, T. H. [> > > >] | |
Heinen, Ernst  [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >] | |
| Simar, L. J. [> > > >] | |
| 1996 | |
| Scandinavian Journal of Immunology | |
| Blackwell Publishing | |
| 43 | |
| 1 | |
| 23-30 | |
| Yes (verified by ORBi) | |
| International | |
| 0300-9475 | |
| 1365-3083 | |
| Oxford | |
| United Kingdom | |
| [en] Antibiotics, Antineoplastic/pharmacology ; Antibodies, Monoclonal/pharmacology ; Antigens, CD40/immunology ; B-Lymphocytes/immunology ; Cell Division ; Cell Separation ; Cells, Cultured ; Child ; Child, Preschool ; DNA Replication ; Dendritic Cells/immunology ; Humans ; Immunoglobulin G/immunology ; Lymphocyte Activation/drug effects ; Mitogens/pharmacology ; Mitomycin/pharmacology ; Palatine Tonsil/cytology ; Staphylococcus aureus/immunology ; Transferrin/immunology | |
| [en] Follicular dendritic cells (FDC) are unique non-lymphoid cells found only in lymph follicles. They play a part in the survival, proliferation and differentiation of B cells. To analyse the influence of FDC on B-lymphocyte proliferation, we isolated them from human tonsils on albumin gradients and treated them with mitomycin C to prevent the multiplication of lymphoid cells harboured in their cytoplasmic evaginations. FDC cultured for 12-16 h remained attached to the substrate; non-adherent cells were carefully eliminated by washing. Purified B cells cultured alone or with contaminant-cleared FDC were maintained for 2 days in the presence or absence of various stimulants, after which tritiated thymidine uptake by these cells was measured. In the absence of activators, FDC did not induce B-cell multiplication. B cells cultured in the presence of FDC exhibited increased 3H-TdR uptake when activated with anti-CD40 MoAb, anti-immunoglobulin MoAb or transferrin, but not when stimulated with Staphylococcus aureus strain Cowan I (SAC) at a given concentration. In the latter case, B-cell proliferation clearly decreased. In control cocultures where mitomycin-C-treated non-adherent cells were used instead of FDC in the presence of the different stimulants, no increase in B-cell proliferation was observed. The results suggest that, inside the germinal centres, FDC modulation of B-cell proliferation depends on the activation state of the B cells and on the stimulant encountered. | |
| http://hdl.handle.net/2268/23312 |
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