[en] Follicular dendritic cells (FDC) are unique non-lymphoid cells found only in lymph follicles. They play a part in the survival, proliferation and differentiation of B cells. To analyse the influence of FDC on B-lymphocyte proliferation, we isolated them from human tonsils on albumin gradients and treated them with mitomycin C to prevent the multiplication of lymphoid cells harboured in their cytoplasmic evaginations. FDC cultured for 12-16 h remained attached to the substrate; non-adherent cells were carefully eliminated by washing. Purified B cells cultured alone or with contaminant-cleared FDC were maintained for 2 days in the presence or absence of various stimulants, after which tritiated thymidine uptake by these cells was measured. In the absence of activators, FDC did not induce B-cell multiplication. B cells cultured in the presence of FDC exhibited increased 3H-TdR uptake when activated with anti-CD40 MoAb, anti-immunoglobulin MoAb or transferrin, but not when stimulated with Staphylococcus aureus strain Cowan I (SAC) at a given concentration. In the latter case, B-cell proliferation clearly decreased. In control cocultures where mitomycin-C-treated non-adherent cells were used instead of FDC in the presence of the different stimulants, no increase in B-cell proliferation was observed. The results suggest that, inside the germinal centres, FDC modulation of B-cell proliferation depends on the activation state of the B cells and on the stimulant encountered.
Butcher EC, Rouse RV, Coffman RL, Nottenbourg CN, Hardy RR, Weissman IC. Surface phenotype of Peyer's patch germinal center cells: implications for the role of germinal centers in B cell differentiation. J Immunol 1982;129:2698-703.
Coico R, Bhogal B, Thorbeke G. Relationship of germinal centers in lymphoid tissue to immunologic memory VI.Transfer of B cell memory with lymph node cells fractionated according to their receptors for peanut agglutinin. J Immunol 1983;131:2254-7.
Gray D, Maclennan ICM, Lane PJL. Virgin B cell recruitment and the lifes span of memory clones during antibody responses to 2,4-dinitrophenyl-hemocyanin. Eur J Immunol 1986;16:641-8.
Tew JG, Mandel TE, Burgess AW. Retention of intact HSA for prolonged periods in the popliteal lymph nodes of specifically immunized mice. Cell Immunol 1979;45:207-12.
Radoux D, Heinen E, Kinet-Denoël C, Tihange E, Simar LJ. Precise localization of antigens on follicular dendritic cells. Cell Tissue Res 1984;235:257-74.
Gray D, Skarvall H. B-cell memory is short-lived in the absence of antigen. Nature 1988;336:70.
Kupp LI, Kosco MH, Schenkein HA, Tew JG. Chemotaxis of germinal center B cells in response to C5a. Eur J Immunol 1991;21:2697-701.
Louis E, Philippet B, Cardos B et al. Intercellular connections between germinal center cells. Mechanisms of adhesion between lymphoid cells and follicular dendritic cells. Acta ORL 1990;43:297-320.
Koopman G, Keehnen RMJ, Lindhout E et al. Adhesion through the LFA-1 (CD11a/CD18)-ICAM-1 (CD54) and the VLA-4 (CD49d)-VCAM-1 (CD106) pathways prevents apoptosis of germinal center B cells. J Immunol 1994;152:3760-7.
Lindhout E, Mevissen MLCM, Kwekkeboom J, Tager JM, Degroot C. Direct evidence that human follicular dendritic cells (FDC) rescue germinal centre B-cells from death by apoptosis. Clin Exp Immunol 1993;91:330-6.
Cormann N, Lesage F, Heinen E, Schaaf-Lafontaine N, Kinet-Denoël C, Simar LJ. Isolation of follicular dendritic cells from human tonsils or adenoids. V. Effect on lymphocyte proliferation and differentiation. Immunol Letters 1986;14:29-35.
Kosco MH, Pflugfelder E, Gray D. Follicular dendritic cell-dependent adhesion and proliferation of B cells in vitro. J Immunol 1992;148:2331-9.
Petrasch SG, Kosco MH, Perezalvarez CJ, Schmitz J, Brittinger G, Proliferation of germinal center B-lymphocytes in-vitro by direct membrane contact with follicular dendritic cells. Immunobiol 1991;183:451-62.
Freedman AS, Munro JM, Rhynhart K. et al. Follicular dendritic cells inhibit human lymphocyte-B proliferation. Blood 1992;80:1284-8.
Marcoty C, Heinen E, Antoine N, Tsunoda R, Simar LJ. Rapid and selective isolation of follicular dendritic cells by low speed centrifugation on discontinuous BSA gradients. Adv Exp Med Biol 1993;329:425-9.
Bosseloir A, Heinen E, Defrance Th, Bouzhazha F, Antoine N, Simar LJ. MoAbs MAS516 and 5B5, two fibroblast markers, recognize human follicular dendritic cells. Immunol Letters 1994;42:49-54.
Lagresle C, Bella C, Defrance Th. Phenotypic and functional heterogeneity of the IgD- B-Cell compartment - identification of two major tonsillar B-cell subsets. Int Immunol 1993;5:1259-68.
Björck P, Paulie S. Inhibition of LFA-1-dependent human B-cell aggregation by CD40 antibodies and interleukin-4 leads to decreased IgE synthesis. Immunol 1993;78:218-25.
Schriever F, Freedman AS, Freeman G et al. Isolated human follicular dendritic cells display a unique antigenic profile. J Exp Med 1989;169:2043-58.
Lederman S, Yellin MJ, Inghirami G, Lee JJ, Knowles DM, Chess L. Molecular interactions mediating lymphocyte-T-B collaboration in human lymphoid follicles - roles of T-cell-B-cell-activating molecule (5c8 antigen) and CD40 in contact-dependent help. J Immunol 1992;149:3817-28.
Heinen E, Lilet-Leclercq C, Mason DY et al. Isolation of follicular dendritic cells from human tonsils and adenoids. II. Immunocytochemical characterization. Eur J Immunol 1984;14: 267-73.
Petrasch S, Perez-Alvarez S, Schmitz J, Kosco M, Brittinger G, Antigenic phenotyping of human follicular dendritic cells isolated from nonmalignant lymphatic tisuue. Eur J Immunol 1990;20:1013-18.
Parmentier HK, Vanderlinden JA, Krijnen J et al. Human follicular dendritic cells - isolation and characteristics in-situ and in suspension. Scand J Immunol 1991;33:441-52.
Koopman G, Parmentier HK, Schuurman HJ, Newman W, Meijer CJ, Pals ST. Adhesion of human B cells to follicular dendritic cells involves both the lymphocyte function-associated antigen 1/intercellular adhesion molecule 1 and. J Exp Med 1991;173:1297-304.
Liu Y, Joshua DE, Williams GT, Smith CA, Gordon J, Maclennan ICM. Mechanism of antigen-driven selection in germinal centres. Nature 1989;342:929-31.
Barrett TB, Shu G, Clark EA. CD40 signaling activates CD11a/CD18 (LFA-1)-mediated adhesion in B cells. J Immunol 1991;146:1722-9.
Clark EA, Grabstein KH, Shu GL. Cultured human follicular dendritic cells -growth characteristics and interactions with lymphocytes-B. J Immunol 1992;148:3327-35.
Banchereau J, De Paoli P, Vallé A, Garcia E, Rousset F. Long-term human B cell lines dependent on interleukin-4 and antibody to CD40. Science 1991;251:70-2.
Theophilopoulos A. Immune complexes in humoral immune responses: suppressive and enhancing effects. Immunol Today 1980;1:1-7.
Lindhout E, Lakeman A, Mevissen MLCM, Degroot C. Functionally active epstein-barr virus-transformed follicular dendritic cell-like cell lines. J Exp Med 1994;179:1173-84.
Iscove NNm Melchers F. Complete replacement of serum by albumin transferrin and soybean lipid in cultures of lipopolysaccharide B lymphocytes. J Exp Med 1978;147:923-33.
Hsu SM, Jaffe ES. Phenotypic expression of B-lymphocytes. 1. Identification with monoclonal antibodies in normal lymphoid tissues. Am J Pathol 1984;114:387-95.
Heinen E, Tsunoda R, Marcoty C et al. Follicular dendritic cells: isolation procedures, short and long term cultures. Adv Exp Med Biol 1993;329:333-8.