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The influence of follicular dendritic cells on B-cell proliferation depends on the activation of B cells and the mitogen used.
Bosseloir, A.; Bouzahzah, F.; Defrance, T. H. et al.
1996In Scandinavian Journal of Immunology, 43 (1), p. 23-30
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Keywords :
Antibiotics, Antineoplastic/pharmacology; Antibodies, Monoclonal/pharmacology; Antigens, CD40/immunology; B-Lymphocytes/immunology; Cell Division; Cell Separation; Cells, Cultured; Child; Child, Preschool; DNA Replication; Dendritic Cells/immunology; Humans; Immunoglobulin G/immunology; Lymphocyte Activation/drug effects; Mitogens/pharmacology; Mitomycin/pharmacology; Palatine Tonsil/cytology; Staphylococcus aureus/immunology; Transferrin/immunology
Abstract :
[en] Follicular dendritic cells (FDC) are unique non-lymphoid cells found only in lymph follicles. They play a part in the survival, proliferation and differentiation of B cells. To analyse the influence of FDC on B-lymphocyte proliferation, we isolated them from human tonsils on albumin gradients and treated them with mitomycin C to prevent the multiplication of lymphoid cells harboured in their cytoplasmic evaginations. FDC cultured for 12-16 h remained attached to the substrate; non-adherent cells were carefully eliminated by washing. Purified B cells cultured alone or with contaminant-cleared FDC were maintained for 2 days in the presence or absence of various stimulants, after which tritiated thymidine uptake by these cells was measured. In the absence of activators, FDC did not induce B-cell multiplication. B cells cultured in the presence of FDC exhibited increased 3H-TdR uptake when activated with anti-CD40 MoAb, anti-immunoglobulin MoAb or transferrin, but not when stimulated with Staphylococcus aureus strain Cowan I (SAC) at a given concentration. In the latter case, B-cell proliferation clearly decreased. In control cocultures where mitomycin-C-treated non-adherent cells were used instead of FDC in the presence of the different stimulants, no increase in B-cell proliferation was observed. The results suggest that, inside the germinal centres, FDC modulation of B-cell proliferation depends on the activation state of the B cells and on the stimulant encountered.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Bosseloir, A.
Bouzahzah, F.
Defrance, T. H.
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Simar, L. J.
Language :
English
Title :
The influence of follicular dendritic cells on B-cell proliferation depends on the activation of B cells and the mitogen used.
Publication date :
1996
Journal title :
Scandinavian Journal of Immunology
ISSN :
0300-9475
eISSN :
1365-3083
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
43
Issue :
1
Pages :
23-30
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 September 2009

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