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Article (Scientific journals)
Ultrastructure of CD57+ cells isolated from human tonsils or blood.
Bouzahzah, F.; Heinen, Ernst; Antoine, Nadine et al.
1993In European Journal of Morphology, 31 (1-2), p. 82-6
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Keywords :
Antibodies, Monoclonal/immunology; Antigens, CD/immunology; Antigens, CD57; Antigens, Differentiation, T-Lymphocyte/immunology; Cell Separation; Child; Humans; Immunosorbent Techniques; Killer Cells, Natural/ultrastructure; Leukocytes, Mononuclear/ultrastructure; Magnetics; Microspheres; Palatine Tonsil/ultrastructure; Rosette Formation; T-Lymphocyte Subsets/ultrastructure
Abstract :
[en] The presence of CD4+, CD57+ T cells in the germinal centers has been reported by several authors. The CD57 antigen is also expressed by natural killer (NK) cells. We purified CD57+ cells from human tonsils and blood by microdissection, rosetting with sheep red blood cells and magnetic cell sorting (MACS) and examined the ultrastructural morphology of these cells. Clear differences were found in cell aspect: blood NK contained large granules which were not found in the tonsillar CD57+ cells. These latter appeared medium-sized and not fully activated. After immunolabeling, the tonsillar CD57+ cells were mainly found in the light zone of the germinal centers.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Bouzahzah, F.
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Antoine, Nadine ;  Université de Liège - ULiège > Département de morphologie et pathologie > Histologie
Bosseloir, A.
Mancini, I.
Simar, L. J.
Language :
English
Title :
Ultrastructure of CD57+ cells isolated from human tonsils or blood.
Publication date :
1993
Journal title :
European Journal of Morphology
ISSN :
0924-3860
eISSN :
1744-4241
Publisher :
Taylor & Francis, Abingdon, United Kingdom
Volume :
31
Issue :
1-2
Pages :
82-6
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 September 2009

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