Monoclonal antibodies to oxytocin: production and characterization

Burgeon, Emmanuel; Chapleur, Michèle; Schoenen, Jean; Remichius, Didier; Legros, Jean-Jacques; Geenen, Vincent; Robert, Françoise

doi:10.1016/0165-5728(91)90045-9

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Article (Scientific journals)

Monoclonal antibodies to oxytocin: production and characterization

Burgeon, Emmanuel; Chapleur, Michèle; Schoenen, Jean et al.

1991 • In Journal of Neuroimmunology, 31, p. 235-244

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https://hdl.handle.net/2268/23243

DOI
10.1016/0165-5728(91)90045-9

PubMed
1995653

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Abstract :

[en] To induce a good immune response to oxytocin (OT) we developed a two-step technique to conjugate OT to thyroglobulin (TG) using glutaraldehyde. We obtained 30 hybridomas recognizing OT-ovalbumin conjugates and 16 stable lines. Three monoclonal antibodies were selected for further characterization. One of them (O13) was found to be very specific for OT using three different techniques (EIA, RIA and ICC); it si directed to the C-terminal tripeptide. The other two probably recongize tyrosine-containing epitope(s) also shared by vasopressin and other related nonapeptides.

Disciplines :

Immunology & infectious disease
Endocrinology, metabolism & nutrition

Author, co-author :

Burgeon, Emmanuel

Chapleur, Michèle

Schoenen, Jean ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Neuro-anatomie

Remichius, Didier

Legros, Jean-Jacques ; Université de Liège - ULiège > Endocrinologie clinique

Geenen, Vincent ; Université de Liège - ULiège > Centre d'immunologie - Embryologie

Robert, Françoise

Language :

English

Title :

Monoclonal antibodies to oxytocin: production and characterization

Publication date :

1991

Journal title :

Journal of Neuroimmunology

ISSN :

0165-5728

eISSN :

1872-8421

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

Pages :

235-244

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Available on ORBi :

since 22 September 2009

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