|Reference : 5-Nucleotidase activity in isolated follicular dendritic cells.|
|Scientific journals : Article|
|Life sciences : Anatomy (cytology, histology, embryology...) & physiology|
|5-Nucleotidase activity in isolated follicular dendritic cells.|
|Heinen, Ernst [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]|
|Kinet-Denoel, C. [> > > >]|
|Simar, L. J. [> > > >]|
|[en] 5'-Nucleotidase ; Animals ; Histocytochemistry ; Lymph Nodes/enzymology/immunology/ultrastructure ; Mice ; Mice, Inbred C57BL ; Microscopy, Electron ; Nucleotidases/metabolism ; Receptors, Fc/metabolism|
|[en] Follicular dendritic cells isolated from mouse lymph nodes were incubated in the presence of AMP to test 5-nucleotidase (5-Nase) activity. Ultrastructural observations showed the presence of 5-Nase on external membranes but also some activity inside the nucleus. 5-Nase was found associated to Fc receptors labelled with homologous immunoglobulins fixed on colloidal gold particles. Lymphocytes and macrophages, found in association with the follicular dendritic cells, were either 5-Nase positive or negative. The hypothetical roles played by 5-Nase in germinal centers are discussed.|
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