[en] Horse-spleen ferritin or bovine serum albumin conjugated to colloidal gold (BSA-gold) were injected subcutaneously in preimmunized mice. In draining lymph nodes both antigens were located in macrophages or between the cytoplasmic processes of follicular dendritic cells (FDC). Some of the antigens remained trapped on FDC until day 31 after injection. Simultaneous injection of both antigens showed that they were located between the infoldings of the same FDC. These cells are thus able to retain at least two different antigens on their surface. The peculiar arrangement of ferritin between the cytoplasmic infoldings suggests that this antigen is fixed on both cell membranes by specific antibodies. The trapped immune complexes could thus stabilize the FDC membrane system. The antigen retention requires the presence of specific antibodies since BSA-gold or ferritin injected without preimmunization were not found between FDC processes. Nonantigenic materials, such as colloidal gold or carbon particles, are not trapped by FDC, except when injected in large amounts. The antigens were trapped on the surface of FDC, however unfrequently in close contact with lymphocytes. FDC might protect lymphocytes against an excess of immune complexes and act as regulators of contacts between lymphocytes and immune complexes.
Baldwin W.M., Cohen N. (1981) A giant cell with dendritic cell properties in spleens of the anuran amphibian Xenopus laevis. Dev Comp Immunol 5:461-473.
.
Chen L.L., Ada J.C., Steinman R.M. (1978) Anatomy of germinal centers in mouse spleen, with special reference to “follicular dendritic cells”. J Cell Biol 77:148-164.
Chen L.L., Frank A., Ada J., Steinman R. (1978) Distribution of horseradish peroxidase (HRP) anti-HRP immune complexes in mouse spleen with special reference to follicular dendritic cells. J Cell Biol 79:184-199.
Cohen S., Vassalli P., Benacerraf B., McCluskey R. (1966) The distribution of antigenic and nonantigenic compounds within draining lymph nodes. Lab Invest 15:1143-1155.
Diener E., Feldmann M. (1972) Relationship between antigen and antibody induced suppression of immunity. Transplant Rev 8:76.
Fossum S. (1980) The architecture of rat lymph nodes. III Distribution of ferritin and colloidal carbon in the draining lymph nodes after food-pad injection. Scand J Immunol 12:433-441.
Gaijl-Peczalska K.J., Fish A.J., Meuwissen H.J., Frommel D., Good R.A. (1969) Localization of immunological complexes fixing C3 in germinal centers of lymph nodes. J Exp Med 130:1367-1374.
Groscurth P. (1980) Non-lymphatic cells in the lymph node cortex of the mouse. I Morphology and distribution of the interdigitating cells and the dendritic reticular cells in the mesenteric lymph node of adult ICR mouse. Pathol Res Pract 169:211-234.
Groscurth P. (1980) Non-lymphatic cells in the lymph node cortex of the mouse. III Interdigitating cells and dendritic reticular cells in the mesenteric lymph node of the homozygotes “nude” (nu/nu) mouse. Pathol Res Pract 169:255-268.
Hanna M.G., Szakal A.K. (1968) Localization of 125I-labelled antigen in germinal centers of mouse spleen: histologic and ultrastructural autoradiographic studies of the secondary immune reaction. J Immunol 101:949-962.
.
Heinen E., Radoux D., Kinet-Denoël C., Simar L.J. (1983) Colloidal gold, a useful marker for antigen localization on follicular dendritic cells. J Immunol Methods 59:361-368.
Herd Z.L., Ada G.L. (1969) The retention of 125I-immunoglobulin, IgG subunits and antigen-antibody complexes in rat footpads and draining lymph nodes. Aust J Exp Biol Med Sci 47:63-77.
Heusermann U., Zurborn K.H., Schroeder L., Stutte H.J. (1980) The origin of the dendritic reticulum cell. An experimental enzymehistochemical and electron microscopic study on the rabbit spleen. Cell Tissue Res 209:279-294.
Humphrey J.G., Frank M.M. (1967) The localization of non-microbial antigens in the draining lymph nodes of tolerant, normal and primed rabbits. Immunol 13:87-94.
Kinet-Denoël C., Heinen E., Radoux D., Simar L.J. (1982) Follicular dendritic cells in lymph nodes after X-irradiation. International Journal of Radiation Biology 42:121-130.
Klaus G.G.B., Humphrey J.H. (1977) The generation of memory cells. I. The role of C3 in the generation of B memory cells. Immunol 33:31-40.
Klaus G.G.B., Humphrey J.H., Kunkl A., Dongworth D.W. (1980) The follicular dendritic cell: its role in antigen presentation in the generation of immunological memory. Immunol Rev 53:3-28.
Kunkl A., Klaus G.G.B. (1981) The generation of memory cells. IV Immunization with antigen-antibody complexes accelerates the development of B-memory cells, the formation of germinal centres and the maturation of antibody affinity in the secondary response. Immunology 43:371-378.
.
Mandel T.E., Phipps R.P., Abbot A., Tew J.G. (1980) The follicular dendritic cell: long term antigen retention during immunity. Immunol Rev 53:29-60.
Mandel T.E., Phipps R.P., Abbot A.P., Tew J.G. (1981) Long-term antigen retention by dendritic cells in the popliteal lymph node of immunized mice. Immunology 43:353-362.
Maruyama K., Masuda T. (1965) Electron microscope observation on the germinal center of the lymph node in guinea pigs sensitized with sheep erythrocytes. Ann Rept Inst Virus Res Kyoto Univ 7:149.
Milanesi S. (1965) Sulla presenza di dispositivi di giunzione tra le cellule dendritiche dei follicoli linfactica del linfonodo. Boll Soc Ital Biol Sper 41:1223-1231.
Morgan E.L., Tempelis C.H. (1977) The role of antigen-antibody complexes in mediating immunologic unresponsiveness in the chicken. J Immunol 119:1293-1298.
Nossal G.J., Ada G.L., Austin C.M. (1965) Antigens in immunity. VIII Localization of 125I labeled antigens in the secondary response. Immunology 9:349-363.
Nossal G.J., Abbot A., Mitchell J., Lummus Z. (1968) Antigens in immunity. XV Ultrastructural features of antigen capture in primary and secondary lymphoid follicles. J Exp Med 127:277-289.
Papamichail M., Gutierrez C., Embling P., Johnson P., Holborow E., Pepys M. (1975) Complement dependence of localisation of aggregated IgG in germinal centres. Scand J Immunol 4:343-347.
Romball C.G., Ulevith R.J., Weigle W.O. (1980) Role of C3 in the regulation of a spheric PFC response in rabbits. J Immunol 124:151-155.
van Rooijen N. (1972) Antigen in the spleen. The non-specificity of the follicles in the process of antigen trapping and the role of antibody. Immunology 22:757-765.
Sakuma H., Asano S., Kojima M. (1981) An ultrastructure study of the primary follicle in the lymph node. Acta Pathol Jpn 31:473-493.
Sordat B., Moser R., Gerber H., Cottier H. (1969) Differentiation pathway within germinal centers of human tonsils. Lymphatic tissue and germinal centers in immune responses , L., FioreDonati, Hanna, Plenum Press, New York; 73-82.
Swartzendruber D.C. (1965) Desmosomes in germinal centers of mouse spleen. Exp Cell Res 40:429-432.
Szakal A.K., Hanna M.G. (1968) The ultrastructure of antigen localization and virus-like particles in mouse spleen germinal centers. Exp Molec Pathol 8:75-83.
Tew J.G., Mandel T. (1978) The maintenance and regulation of serum antibody levels: evidence indicating a role for antigen retained in lymphoid follicles. J Immunol 120:1063-1069.
Tew J.G., Mandel T.E., Burgess A.W. (1979) Retention of intact HSA for prolonged periods in the popliteal lymph nodes of specifically immunized mice. Cell Immunol 45:207-212.
Tew J.G., Mandel T.E., Miller G.A. (1979) Immune retention: immunological requirements for maintaining an easily degradable antigen in vivo. Aust J Exp Biol Med Sc 57:401-414.
Thorbeke G.T., Romano T.J., Lerman S.P. (1974) Regulatory mechanisms in proliferation and differentiation of lymphoid tissue with particular reference to germinal center development. Prog Immunol Stand II 3:25-34.
Tsunoda R., Yaginuma Y., Kojima M. (1980) Immunocytological studies on the constituent cells of the secondary nodules in human tonsils. Acta Pathol Jpn 30:33-57.
Unanue E.R. (1972) The regulatory role of macrophages in antigenic stimulation. Adv Immunol 15:95-165.
White R.G., French V.I., Stark J.M. (1970) A study of the localization of a protein antigen in the chicken spleen and its relation to the formation of germinal centres. J Med Microbiol 3:65-71.
