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Lymphoid cell apoptosis induced by trophoblastic cells: a model of active foeto-placental tolerance
Coumans, Bernard; Thellin, Olivier; Zorzi, Willy et al.
1999In Journal of Immunological Methods, 224 (1-2), p. 185-196
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Abstract :
[en] To test the hypothesis that CD95-L (Fas-L) present on trophoblastic cells plays a part in establishing foeto-placental tolerance by inducing apoptosis of immune defence cells, we cocultured trophoblasts with lymphoid cells and scored the frequency of cell death in these cultures. We prepared human trophoblastic cells from term placentas removed by C-section and placed them in culture for 48 h before introducing the lymphoid cells. We added Jurkat cells, a CD3 + lymphoid cell line, or purified T cells from human blood to the cultured trophoblasts and monitored apoptosis by electron microscopy and flow cytometry after TUNEL or annexin V labelling. The frequency of cell death in the CD3 + cell population was higher when the lymphoid cells were cocultured with trophoblastic cells than when they were cultured alone. This frequency increased with time but was reduced when anti-CD95-L antibodies were added to the culture medium. Cell death was less frequent in the lymphoid cell population when trophoblasts were replaced with human fibroblasts not expressing CD95-L. (C) 1999 Elsevier Science B.V. All rights reserved.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Coumans, Bernard ;  Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Thellin, Olivier ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Zorzi, Willy ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Mélot, France ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine)
Bougoussa, M.
Melen-Lamalle, Laurence ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Zorzi, Danièle ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Hennen, Georges ;  Centre Hospitalier Universitaire de Liège - CHU > Endocrinologie clinique
Igout, Ahmed ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Language :
English
Title :
Lymphoid cell apoptosis induced by trophoblastic cells: a model of active foeto-placental tolerance
Publication date :
1999
Journal title :
Journal of Immunological Methods
ISSN :
0022-1759
eISSN :
1872-7905
Publisher :
Elsevier Science
Volume :
224
Issue :
1-2
Pages :
185-196
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 September 2009

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