Reference : Lymphoid cell apoptosis induced by trophoblastic cells: a model of active foeto-placenta...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/23182
Lymphoid cell apoptosis induced by trophoblastic cells: a model of active foeto-placental tolerance
English
Coumans, Bernard mailto [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
Thellin, Olivier mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Zorzi, Willy mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Mélot, France mailto [Université de Liège - ULg > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine) >]
Bougoussa, M. [> > > >]
Melen-Lamalle, Laurence mailto [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Zorzi, Danièle mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Hennen, Georges [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique >]
Igout, Ahmed mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Heinen, Ernst mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
1999
Journal of Immunological Methods
Elsevier Science
224
1-2
185-196
Yes (verified by ORBi)
International
0022-1759
[en] To test the hypothesis that CD95-L (Fas-L) present on trophoblastic cells plays a part in establishing foeto-placental tolerance by inducing apoptosis of immune defence cells, we cocultured trophoblasts with lymphoid cells and scored the frequency of cell death in these cultures. We prepared human trophoblastic cells from term placentas removed by C-section and placed them in culture for 48 h before introducing the lymphoid cells. We added Jurkat cells, a CD3 + lymphoid cell line, or purified T cells from human blood to the cultured trophoblasts and monitored apoptosis by electron microscopy and flow cytometry after TUNEL or annexin V labelling. The frequency of cell death in the CD3 + cell population was higher when the lymphoid cells were cocultured with trophoblastic cells than when they were cultured alone. This frequency increased with time but was reduced when anti-CD95-L antibodies were added to the culture medium. Cell death was less frequent in the lymphoid cell population when trophoblasts were replaced with human fibroblasts not expressing CD95-L. (C) 1999 Elsevier Science B.V. All rights reserved.
http://hdl.handle.net/2268/23182

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