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| Reference : Lymphoid cell apoptosis induced by trophoblastic cells: a model of active foeto-placenta... |
| Scientific journals : Article | |||
| Life sciences : Anatomy (cytology, histology, embryology...) & physiology | |||
| http://hdl.handle.net/2268/23182 | |||
| Lymphoid cell apoptosis induced by trophoblastic cells: a model of active foeto-placental tolerance | |
| English | |
Coumans, Bernard  [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >] | |
| Thellin, Olivier [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >] | |
| Zorzi, Willy [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >] | |
| Mélot, France [Université de Liège - ULg > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine) >] | |
| Bougoussa, M. [> > > >] | |
| Melen-Lamalle, Laurence [Université de Liège - ULg > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >] | |
| Zorzi, Danièle [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >] | |
| Hennen, Georges [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique >] | |
| Igout, Ahmed [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >] | |
| Heinen, Ernst [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >] | |
| 1999 | |
| Journal of Immunological Methods | |
| Elsevier Science | |
| 224 | |
| 1-2 | |
| 185-196 | |
| International | |
| 0022-1759 | |
| [en] To test the hypothesis that CD95-L (Fas-L) present on trophoblastic cells plays a part in establishing foeto-placental tolerance by inducing apoptosis of immune defence cells, we cocultured trophoblasts with lymphoid cells and scored the frequency of cell death in these cultures. We prepared human trophoblastic cells from term placentas removed by C-section and placed them in culture for 48 h before introducing the lymphoid cells. We added Jurkat cells, a CD3 + lymphoid cell line, or purified T cells from human blood to the cultured trophoblasts and monitored apoptosis by electron microscopy and flow cytometry after TUNEL or annexin V labelling. The frequency of cell death in the CD3 + cell population was higher when the lymphoid cells were cocultured with trophoblastic cells than when they were cultured alone. This frequency increased with time but was reduced when anti-CD95-L antibodies were added to the culture medium. Cell death was less frequent in the lymphoid cell population when trophoblasts were replaced with human fibroblasts not expressing CD95-L. (C) 1999 Elsevier Science B.V. All rights reserved. | |
| http://hdl.handle.net/2268/23182 |
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