Reference : Culture of Gingival Fibroblasts on Bioabsorbable Regenerative Materials in Vitro
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/23180
Culture of Gingival Fibroblasts on Bioabsorbable Regenerative Materials in Vitro
English
Simain-Sato, Franklin mailto [Centre Hospitalier Universitaire de Liège - CHU > > Médecine dentaire >]
Lahmouzi, Jamila mailto [Centre Hospitalier Universitaire de Liège - CHU > > Médecine dentaire >]
Kalykakis, G. K. [> > > >]
Heinen, Ernst mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Defresne, Marie-Paule mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
De Pauw, M. C. [> > > >]
Grisar, Thierry mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique]
Legros, Jean-Jacques mailto [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
Legrand, Roman mailto [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Oct-1999
Journal of Periodontology
70
10
1234-9
Yes (verified by ORBi)
International
0022-3492
[en] BACKGROUND: The use of membranes in guided tissue regeneration (GTR) can limit the apical migration of gingival cells and favor the establishment of new attachment by periodontal ligament fibroblasts. However, gingival recession during healing following GTR has been described as a frequent complication. The purpose of this study was to determine if gingival fibroblasts are affected by the composition of the bioabsorbable membranes used in mucogingival surgery. METHODS: Two type of bioabsorbable regenerative materials were used as cell carriers. Wistar rat gingival fibroblasts (RGF) were obtained from attached gingiva, cut into small fragments, and placed in culture dishes. When confluent, cells were detached using trypsin and identified as "first transferred cells" (P1). At the third passage (P3), cell count, trypan blue exclusion test, acid phosphatase activity, DNA synthesis, phase contrast microscopy, and scanning electron microscopy were performed. The cells were then placed in wells containing the membranes and incubated for 72 hours. RESULTS: When examined under microscopy, the control wells (without membranes) showed one cell type with the elongated appearance characteristic of fibroblasts. The wells with membranes showed an altered cell morphology with a high proportion of cell fragments regardless of the type of membrane used. CONCLUSIONS: These results suggest that cell carrier membranes could affect RGF morphology and thus alter gingival tissue healing following GTR.
http://hdl.handle.net/2268/23180

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