Reference : Intrinsic pro-angiogenic status of cystic fibrosis airway epithelial cells
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/23171
Intrinsic pro-angiogenic status of cystic fibrosis airway epithelial cells
English
Verhaeghe, Catherine mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > GIGA-R : Génétique générale et humaine >]
Tabruyn, Sébastien mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Oury, Cécile mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Génétique générale et humaine >]
Bours, Vincent mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Génétique générale et humaine]
Griffioen, Arjan W [> > > >]
2007
Biochemical and Biophysical Research Communications
Academic Press
356
3
745-749
Yes (verified by ORBi)
International
0006-291X
1090-2104
San Diego
CA
[en] angiogenesis ; Cell Line ; cystic fibrosis ; lung inflammation ; VEGF ; endothelial cells ; epithelial cells
[en] Cystic fibrosis is a common genetic disorder characterized by a severe lung inflammation and fibrosis leading to the patient's death. Enhanced angiogenesis in cystic fibrosis (CF) tissue has been suggested, probably caused by the process of inflammation, as similarly described in asthma and chronic bronchitis. The present study demonstrates an intrinsic pro-angiogenic status of cystic fibrosis airway epithelial cells. Microarray experiments showed that CF airway epithelial cells expressed several angiogenic factors such as VEGF-A, VEGF-C, bFGF, and PLGF at higher levels than control cells. These data were confirmed by real-time quantitative PCR and, at the protein level, by ELISA. Conditioned media of these cystic fibrosis cells were able to induce proliferation, migration and sprouting of cultured primary endothelial cells. This report describes for the first time that cystic fibrosis epithelial cells have an intrinsic angiogenic activity. Since excess of angiogenesis is correlated with more severe pulmonary disease, our results could lead to the development of new therapeutic applications. (c) 2007 Elsevier Inc. All rights reserved.
http://hdl.handle.net/2268/23171
also: http://hdl.handle.net/2268/77523
10.1016/j.bbrc.2007.02.166

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