ORBi: Jacobs Nathalie - Distinct T cell subsets and cytokine production in cultures derived from transformation zone and squamous intraepithelial lesion biopsies of the uterine cervix.

Reference : Distinct T cell subsets and cytokine production in cultures derived from transformation ...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Immunology & infectious disease Human health sciences : Oncology
	To cite this reference:	http://hdl.handle.net/2268/22999

Title :	Distinct T cell subsets and cytokine production in cultures derived from transformation zone and squamous intraepithelial lesion biopsies of the uterine cervix.
Language :	English
Author, co-author :	Jacobs, Nathalie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Renard, Isabelle [> > > >]
	Al-Saleh, Walid [> > > >]
	Hubert, Pascale [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Doyen, Jean [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
	Kedzia, Witold [> > > >]
	Boniver, Jacques [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
	Delvenne, Philippe [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Publication date :	2003
Journal title :	American Journal of Reproductive Immunology
Publisher :	Blackwell Munksgaard
Volume :	49
Issue/season :	1
Pages :	6-13
Audience :	International
ISSN :	1046-7408
City :	Copenhagen
Country :	Denmark
Keywords :	[en] Biopsy ; Cell Transformation, Neoplastic/metabolism ; Cervical Intraepithelial Neoplasia/immunology/pathology ; Cervix Uteri/cytology/metabolism ; Cytokines/metabolism ; Epithelium/metabolism ; Female ; Humans ; Receptors, Antigen, T-Cell/metabolism ; Receptors, Interleukin-2/metabolism ; Stromal Cells/metabolism ; T-Lymphocyte Subsets/metabolism ; Uterine Cervical Neoplasms/immunology/pathology
Abstract :	[en] PROBLEM: The characterization of lymphocytes issued from squamous intraepithelial lesions (SIL) and from the transformation zone (TZ), where the majority of SIL occur, is important to understand the role of immunity in SIL development. METHOD OF STUDY: We compared lymphocyte populations of the TZ and SIL with those of normal exocervix, using a technique allowing for the isolation of lymphocytes, either from the epithelium or from the underlying stroma of small biopsies. RESULTS: The majority of cells derived from the epithelium of all biopsies were CD8+ T cells. Some SIL-derived cultures were characterized by an increased proportion of activated TCRgammadelta+. The production of the immunosuppressive cytokine IL10 was significantly higher in lymphocyte cultures from the normal TZ in comparison with the exocervix. A decreased percentage of effector T cells was observed in cultures derived from the stroma of normal TZ (TCRgammadelta+) or SIL (CD8+) in comparison with the exocervix. CONCLUSIONS: Our results suggest that a low proportion of effector T cells and IL10 production could contribute to the predisposition of the TZ to the development of SIL and to the progression of SIL to cervical cancer.
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Centre de Recherche Interuniversitaire en Vaccinologie (Région Wallonne et GSK) ; EU BIO4-CT98-0097 contract
Target :	Researchers ; Students
Permalink :	http://hdl.handle.net/2268/22999

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