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| Reference : Neurohypophysial Peptides Stimulate the Phosphorylation of Pre-T Cell Focal Adhesion Kin... |
| Scientific journals : Article | |||
| Human health sciences : Endocrinology, metabolism & nutrition Human health sciences : Immunology & infectious disease | |||
| http://hdl.handle.net/2268/22908 | |||
| Neurohypophysial Peptides Stimulate the Phosphorylation of Pre-T Cell Focal Adhesion Kinases | |
| English | |
Martens, Henri  [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Embryologie >] | |
| Kecha, O. [> > > >] | |
| Charlet-Renard, C. [> > > >] | |
| Defresne, Marie-Paule [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >] | |
| Geenen, Vincent [Université de Liège - ULg > > Centre d'immunologie >] | |
| Apr-1998 | |
| Neuroendocrinology | |
| 67 | |
| 4 | |
| 282-9 | |
| International | |
| 0028-3835 | |
| [en] Thymic oxytocin (OT) behaves as a cryptocrine signal targeted at the outer surface of thymic epithelial cell plasma membrane from where OT is able to interact with neurohypophysial peptide receptors expressed by pre-T cells. Immature T cells bear a receptor of the V1 subtype, while OT receptors are predominantly expressed by cytotoxic CD8+ lymphocytes. In both T cell types, neurohypophysial peptide receptors transduce OT via the phosphoinositide pathway. Protein tyrosine phosphorylation is an early event of T cell activation. Western blots of murine pre-T cells (RL12-NP line) proteins probed with anti-phosphotyrosine (PY-20) revealed a great number of proteins the phosphorylation of which increased either with OT or vasopressin treatment. Two were immunoprecipitated with anti-focal adhesion kinase (FAK) mAb 2A7 and were identified one as p125FAK and the other as a coprecipitating 130-kDa protein. The p125FAK is connected to the Ras/MAPK pathway and is also implicated in TCR/CD3 signalling in T cell. Another protein phosphorylated by OT in RL12-NP was identified as paxillin, a 68-kDa protein localised at focal adhesion sites and associated with p 125FAK. These results indicate that phosphorylation of focal adhesion kinase may be induced in pre-T cell by thymic OT. | |
| http://hdl.handle.net/2268/22908 |
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