ORBi: Girard Geneviève - Pip, a novel activator of phenazine biosynthesis in Pseudomonas chlororaphis PCL1391

Reference : Pip, a novel activator of phenazine biosynthesis in Pseudomonas chlororaphis PCL1391


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/2268/22675

Title :	Pip, a novel activator of phenazine biosynthesis in Pseudomonas chlororaphis PCL1391
Language :	English
Author, co-author :	Girard, Geneviève [ > > ]
	Barends, S. [ > > ]
	Rigali, Sébastien [Université de Liège - ULg > Département des sciences de la vie > Département des sciences de la vie >]
	van Rij, E. T. [ > > ]
	Lugtenberg, B. J. [ > > ]
	Bloemberg, G. V. [ > > ]
Publication date :	2006
Journal title :	Journal of Bacteriology
Publisher :	American Society for Microbiology (ASM)
Volume :	188
Issue/season :	23
Pages :	8283-8293
Audience :	International
ISSN :	0021-9193
e-ISSN :	1098-5530
City :	Washington
Country :	DC
Keywords :	[en] Phenazine ; Pseudomonas ; Quorum sensing ; TetR family
Abstract :	[en] Secondary metabolites are important factors for interactions between bacteria and other organisms. Pseudomonas chlororaphis PCL1391 produces the antifungal secondary metabolite phenazine-1-carboxamide (PCN) that inhibits growth of Fusarium oxysporum f. sp. radius lycopersici the causative agent of tomato foot and root rot. Our previous work unraveled a cascade of genes regulating the PCN biosynthesis operon, phzABCDEFGH. Via a genetic screen, we identify in this study a novel TetR/AcrR regulator, named Pip (phenazine inducing protein), which is essential for PCN biosynthesis. A combination of a phenotypical characterization of a pip mutant, in trans complementation assays of various mutant strains, and electrophoretic mobility shift assays identified Pip as the fifth DNA-binding protein so far involved in regulation of PCN biosynthesis. In this regulatory pathway, Pip is positioned downstream of PsrA (Pseudomonas sigma factor regulator) and the stationary-phase sigma factor RpoS, while it is upstream of the quorum-sensing system PhzI/PhzR. These findings provide further evidence that the path leading to the expression of secondary metabolism gene clusters in Pseudomonas species is highly complex.
Permalink :	http://hdl.handle.net/2268/22675

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