Article (Scientific journals)
Pip, a novel activator of phenazine biosynthesis in Pseudomonas chlororaphis PCL1391
Girard, Geneviève; Barends, S.; Rigali, Sébastien et al.
2006In Journal of Bacteriology, 188 (23), p. 8283-8293
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Keywords :
Phenazine; Pseudomonas; Quorum sensing; TetR family
Abstract :
[en] Secondary metabolites are important factors for interactions between bacteria and other organisms. Pseudomonas chlororaphis PCL1391 produces the antifungal secondary metabolite phenazine-1-carboxamide (PCN) that inhibits growth of Fusarium oxysporum f. sp. radius lycopersici the causative agent of tomato foot and root rot. Our previous work unraveled a cascade of genes regulating the PCN biosynthesis operon, phzABCDEFGH. Via a genetic screen, we identify in this study a novel TetR/AcrR regulator, named Pip (phenazine inducing protein), which is essential for PCN biosynthesis. A combination of a phenotypical characterization of a pip mutant, in trans complementation assays of various mutant strains, and electrophoretic mobility shift assays identified Pip as the fifth DNA-binding protein so far involved in regulation of PCN biosynthesis. In this regulatory pathway, Pip is positioned downstream of PsrA (Pseudomonas sigma factor regulator) and the stationary-phase sigma factor RpoS, while it is upstream of the quorum-sensing system PhzI/PhzR. These findings provide further evidence that the path leading to the expression of secondary metabolism gene clusters in Pseudomonas species is highly complex.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
Girard, Geneviève
Barends, S.
Rigali, Sébastien  ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
van Rij, E. T.
Lugtenberg, B. J.
Bloemberg, G. V.
Language :
English
Title :
Pip, a novel activator of phenazine biosynthesis in Pseudomonas chlororaphis PCL1391
Publication date :
2006
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Volume :
188
Issue :
23
Pages :
8283-8293
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 September 2009

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