Reference : Influence of the nature of the electrolyte on the chiral separation of basic compounds i...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/22539
Influence of the nature of the electrolyte on the chiral separation of basic compounds in nonaqueous capillary electrophoresis using heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-cyclodextrin
English
Servais, Anne-Catherine mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Chiap, Patrice mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Dewé, Walthère [Eli Lilly and Company > Lilly Development Centre > Statistical and Mathematical Sciences > >]
Hubert, Philippe mailto [Université de Liège - ULg > > Chimie analytique >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
11-Mar-2005
Journal of Chromatography. A
Elsevier Science Bv
1068
1
143-150
Yes (verified by ORBi)
International
0021-9673
Amsterdam
[en] nonaqueous capillary electrophoresis ; cyclodextrins ; enantiomer separation ; basic drugs ; background electrolyte composition
[en] The influence on the enantiomeric resolution of the nature of the cationic BGE component (sodium, ammonium or potassium) and that of the anionic component (chloride, formate, methanesulfonate or camphorsulfonate) as well as the concentration of heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-cyclodextrin (HDMS-beta-CD), the selected chiral selector, was studied in nonaqueous capillary electrophoresis (NACE). For this purpose, two D-optimal designs with 33 and 26 experimental points were applied. Three beta-blockers (atenolol, celiprolol and propranolol) and three local anesthetics (bupivacaine, mepivacaine and prilocaine) were selected as basic model compounds. Both cationic and anionic BGE components were found to have a deep impact on the enantiomeric resolution of the investigated analytes but it is the cationic component that has shown the strongest influence. Indeed, in some cases, the change of the latter led to a complete loss of enantioresolution. Based on the observed results, two NACE systems were recommended, namely ammonium formate and potassium camphorsulfonate in a methanolic solution containing HDMS-beta-CD and acidified with formic acid, in order to separate efficiently the enantiomers of basic drugs. (c) 2004 Elsevier B.V. All rights reserved.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq
http://hdl.handle.net/2268/22539

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
JCA 2005 (vol 1068, pp 143-150).docPublisher postprint459.5 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.