Reference : Improved detection of chromosomal abnormalities in chronic lymphocytic leukemia by conve...
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/22263
Improved detection of chromosomal abnormalities in chronic lymphocytic leukemia by conventional cytogenetics using CpG oligonucleotide and interleukin-2 stimulation: A Belgian multicentric study.
English
Put, Natalie [> > > >]
Konings, Peter [> > > >]
Rack, Katrina [> > > >]
Jamar, Mauricette mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Van Roy, Nadine [> > > >]
Libouton, Jeanne*-Marie [> > > >]
Vannuffel, Pascal [> > > >]
Sartenaer, Daniel [> > > >]
Ameye, Genevieve [> > > >]
Speleman, Frank [> > > >]
Herens, Christian mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Poirel, Helene A [> > > >]
Moreau, Yves [> > > >]
Hagemeijer, Anne [> > > >]
Vandenberghe, Peter [> > > >]
Michaux, Lucienne [> > > >]
2009
Genes, Chromosomes & Cancer
Wiley Liss, Inc.
48
10
843-53
Yes (verified by ORBi)
International
1045-2257
1098-2264
New York
NY
[en] Adult ; Aged ; Aged, 80 and over ; Cell Proliferation/drug effects ; Chromosome Aberrations ; Chromosome Banding ; Cytogenetics/methods ; Data Interpretation, Statistical ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Interleukin-2/pharmacology ; Karyotyping/methods ; Leukemia, Lymphocytic, Chronic, B-Cell/genetics/pathology ; Male ; Middle Aged ; Oligonucleotides/pharmacology ; Prospective Studies ; Retrospective Studies ; Translocation, Genetic ; Tumor Cells, Cultured
[en] We performed a multicentric study to assess the impact of two different culture procedures on the detection of chromosomal abnormalities in 217 consecutive unselected cases with chronic lymphocytic leukemia (CLL) referred for routine analysis either at the time of diagnosis (n = 172) or during disease evolution (n = 45). Parallel cultures of peripheral blood or bone marrow were set up with the addition of either the conventional B-cell mitogen 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or a combination of CpG oligonucleotide (CpG) and interleukin-2 (IL-2). Cytogenetic analyses were performed on both cultures. Clonal abnormalities were identified in 116 cases (53%). In 78 cases (36%), the aberrant clone was detected in both cultures. Among these, the percentages of aberrant metaphases were similar in both conditions in 17 cases, higher in the CpG/IL-2 culture in 43 cases, and higher in the TPA culture in 18 cases. Clonal aberrations were detected in only one culture, either in CpG/IL-2 or TPA in 33 (15%) and 5 (2%) cases, respectively. Taken together, abnormal karyotypes were observed in 51% with CpG/IL-2 and 38% with TPA (P < 0.0001). Application of FISH (n = 201) allowed the detection of abnormalities not visible by conventional cytogenetic analysis in 80 cases: del(13q) (n = 71), del(11q) (n = 5), +12 (n = 2), del(14q) (n = 1), and del(17p) (n = 1). In conclusion, our results confirm that CpG/IL-2 stimulation increases the detection rate of chromosomal abnormalities in CLL compared with TPA and that further improvement can be obtained by FISH. However, neither conventional cytogenetics nor FISH detected all aberrations, demonstrating the complementary nature of these techniques.
http://hdl.handle.net/2268/22263
10.1002/gcc.20691
(c) 2009 Wiley-Liss, Inc.

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