[en] Two methods were tested for suppressing the depressive effect of N-free diets on the digestive secretions in pigs: the blood perfusion of amino acids (AA) or the peptide alimentation method. In the latter, enzymically hydrolysed casein (EHC), composed of oligopeptides and free AA, was used as the source of nitrogen. The unabsorbed dietary N molecules were discarded from the ileal digesta by ultrafiltration or gel filtration, assuming that the endogenous fraction did not
contain significant amounts of small molecules. The AA supply by blood perfusion had no effect on the ileal endogenous AA losses (8.0 g AA kg-' DM intake) in growing pigs (k 50 kg), compared with the N-free diet alone (8.3 g), whereas the EHC supplementation significantly increased them (18.0 8). The increase was due to both endogenous and dietary N. The presence of unabsorbed dietary AA in the ileal digesta was confirmed by the AA profile of the soluble molecules with a very low molecular mass (< 3 kDa), which was close to that of EHC. Both ultrafiltration (cut-offs of 3 or 10 kDa) and gel filtration methods, utilised to discard the remaining dietary molecules, also eliminated a significant proportion of endogenous AA.
Leterme, Pascal; Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > Unité de Zootechnie
Monmart, Thierry; Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > Unité deZootechnie
Thewis, André ; Université de Liège - ULiège > Gembloux Agro-Bio Tech
Morandi, Pierre; Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > Unité de Zootechnie
Language :
English
Title :
Effect Of Oral And Parenteral N Nutrition Vs N-Free Nutrition On The Endogenous Amino Acid Flow At The Ileum Of The Pig
Publication date :
1996
Journal title :
Journal of the Science of Food and Agriculture
ISSN :
0022-5142
eISSN :
1097-0010
Publisher :
John Wiley & Sons, Inc, Chichester, United Kingdom
Volume :
71
Issue :
2
Pages :
265-271 265-271
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
IRSIA - Institut pour l'Encouragement de la Recherche Scientifique dans l'Industrie et l'Agriculture [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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