[en] Contents Thirteen female Bedouin goats living in arid land of Algeria Sahara desert were used in this study. These goats were pregnant but they sustained an abortion because of unidentified causes. None of the goats showed any signs of general disease. Plasma concentrations of caprine pregnancy-associated glycoproteins (cPAGs) and progesterone (P4) were determined during pregnancy using radioimmunoassay. The cPAGs concentration was undetectable (<0.8 ng/ml) throughout the first 2 weeks of gestation. From week 3 after mating, cPAGs concentration was detectable with significant individual variations (p < 0.05) reaching a maximum secretion (436.1 ng/ml). Throughout gestation, cPAGs concentration remained relatively constant but decreased few days before abortion, on an average of 9.2 +/- 1.2 days (n = 11), except for two females where the concentrations decreased later (1-2 days before abortion). One or two peaks of cPAGs concentrations (in 4/13 and in 9/13 females, respectively) have been measured few weeks before abortion (77-124 days after mating), when a decline of cPAGs was detected. The P4 concentration increased after mating, and was high from the first week till the end of pregnancy. The P4 concentration (9.1 +/- 0.9 ng/ml) decreased rapidly (<0.5 ng/ml) after 4 +/- 0.7 days (n = 6) or 9.4 +/- 1.6 days (n = 7) before abortion. A positive relationship (p < 0.01) was found between P4 and cPAGs concentrations during gestation. Results indicate that cPAGs and P4 measurements can be used for monitoring gestation and for abortion prediction.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Charallah, Salima; Research Laboratory of Arid Lands, Faculty of Biology, USTHB, Algiers, Algeria
Amirat, Z.; Research Laboratory of Arid Lands, Faculty of Biology, USTHB, Algiers, Algeria
Sulon, Joseph; Université de Liège - ULiège
Khammar, F.; Research Laboratory of Arid Lands, Faculty of Biology, USTHB, Algiers, Algeria
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Pregnancy-Associated Glycoprotein and Progesterone Concentrations during Pregnancy Failure in Bedouin Goat from the Southwest of Algeria.
Anous MR, Mourad M, 1993: Crossbreeding effects on reproductive traits of does and growth and carcass traits of kids. Small Rum Res 12, 141-149.
Batalha ES, Sulon J, Figueiredo JR, Beckers JF, Martins R, Silva LDM, 2001a: Relationship between maternal concentrations of caprine pregnancy-associated glycoprotein in Alpine goats and the number of fetuses using a homologous radioimmunoassay. Small Rum Res 42, 105-109.
Batalha ES, Sulon J, Figueiredo JR, Beckers JF, Espeschit CJB, Martins R, Silva LDM, 2001b: Plasma profile of pregnancy associated glycoprotein (PAG) in pregnant Alpine goats using two radioimmunoassay (RIA) systems. Small Rum Res 42, 111-118.
Beckers JF, Wouters-Ballmann P, Ectors F, Derivaux J, 1978: Oestrus induction in heifers in the functional anoestrus. Ann Med Vet 122, 579-605.
Benitez-Ortiz W, 1992: Diagnostic de Gestation et Étude de la Mortalité Embryonnaire Chez les Ruminants par Dosage de la Pregnancy-Associated Glycoprotein (PAG). Thèse de PhD. Institut de Médecine Tropicale Prince Léoplod, Antwerpen, Belgique.
Chentouf M, El Amiri B, Sulon J, Beckers JF, Kirschvink N, Boulanouar B, Bister JL, 2008: Pregnancy-associated glycoprotein secretion in North Moroccan goats. Reprod Domest Anim 43, 696-700.
Dosogne H, Massart-Leen AM, Burvenich C, 2000: Immunological aspects of pregnancy-associated glycoproteins. Adv Exp Med Biol 480, 295-305.
Engeland IV, Waldeland H, Kindahl H, Ropstad E, Andresen O, 1996: Effect of Toxoplasma gondii infection on the development and on endocrine foetal-placental function in the goat. Vet Parasitol 67, 61-74.
Engeland IV, Waldeland H, Ropstad E, Kindahl H, Andresen O, 1997: Effect of experimental infection with Listeria monocytogenes on the development of pregnancy and on concentrations of progesterone, oestrone sulphate and 15-ketodihydro-PGF2α in the goat. Anim Reprod Sci 45, 311-327.
Engeland IV, Waldeland H, Andresen O, Loken T, Bjorkman C, Bjerkas I, 1998: Fetal loss in dairy goats: an epidemiological study in 22 herds. Small Rum Res 48, 30-37.
Faye D, Sulon J, Kane Y, Beckers JF, Leak S, Kaboret Y, Sousa NM, Losson B, Geerts S, 2004: Effects of an experimental Trypanosoma congolense infection on the reproductive performance of West African Dwarf goats. Theriogenology 62, 1438-1451.
Garbayo JM, Remy B, Alabart JL, Folch J, Wattiez R, Falmagne P, Beckers JF, 1998: Isolation and partial characterization of a pregnancy-associated Glycoprotein family from the goat placenta. Biol Reprod 58, 109-115.
Garbayo JM, Green JA, Manikkam M, Beckers JF, Kiesling DO, Ealy AD, Roberts RM, 2000: Caprine pregnancy-associated glycoproteins (PAG): their cloning, expression, and evolutionary relationship to other PAG. Mol Reprod Dev 57, 311-322.
Garbayo JM, Serrano B, Lopez-Gatius F, 2008: Identification of novel pregnancy-associated glycoproteins (PAG) expressed by peri-implantation conceptus of domestic ruminants. Anim Reprod Sci 103, 120-134.
Gonzalez F, Sulon J, Garbayo JM, Batistita M, Cabrera F, Calero P, Gracia A, Beckers JF, 1999: Early pregnancy diagnosis in goats by determination of pregnancy-associated glycoprotein concentrations in plasma samples. Theriogenology 52, 717-725.
Gonzalez F, Sulon J, Garbayo JM, Batista M, Cabrera F, Calero PO, Gracia A, Beckers JF, 2000: Secretory profiles of pregnancy-associated glycoproteins at different stages of pregnancy in the goat. Reprod Domest Anim 35, 79-82.
Gonzalez F, Cabrera F, Batista M, Rodriguez N, Alamo D, Sulon J, Beckers JF, Gracia A, 2004: A comparison of diagnosis of pregnancy in the goat via transrectal ultrasound scanning, progesterone, and pregnancy-associated glycoprotein assays. Theriogenology 62, 1108-1115.
Goossens B, Osaer S, Kora S, 1997: Long term effects of an experimental infection with Trypanosoma congolense on reproductive performance of trypanotolerant Djallonke ewe and West African Dwarf does. Res Vet Sci 63, 169-173.
Green JA, Xie S, Quan X, Bao B, Gan X, Mathialagan N, Beckers JF, Roberts RM, 2000: Pregnancy-associated bovine and ovine glycoproteins exhibit spatially and temporally distinct expression patterns during pregnancy. Biol Reprod 62, 1624-1631.
Guilbaut LA, Beckers JF, Lapierre S, Zoli AP, Benitez-Ortiz W, Roy GL, 1991: Peripartum concentration of placental protein hormones (hPL and bPAG) on holstein and hereford recipients carrying pubered Holstein fetuses. Theriogenology 35(Suppl. 1), 208, (Abstract).
Guillomot M, 1995: Cellular interactions during implantation in domestic ruminants. J Reprod Fert 49(Suppl.), 39-51.
Guruprasad K, Blundell TL, Xie S, Green J, Szafranska B, Nagel RJ, McDowell K, Baker CB, Roberts RM, 1996: Comparative modelling and analysis of amino acid substitutions suggests that family of pregnancy-associated glycoporteins includes both active and inactive aspartic proteinases. Protein Eng 9, 849-856.
Humblot P, De Montigny G, Jeanguyot N, Tetedoie F, Payen B, Thibier M, Sasser RG, 1990: Pregnancy-specific protein B and progesterone concentrations in French Alpine goats throughout gestation. J Reprod Fertil 89, 205-212.
Hussain Q, Ropstad E, Andresen O, 1996: Effects of type and quality of roughage and energy level on plasma progesterone levels in pregnant goats. Small Rum Res 21, 113-120.
Jarrell VL, Dziuk W, 1991: Effect of number of corpora lutea and fetuses on concentrations of progesterone in blood of goats. J Anim Sci 69, 770-773.
Jourdain E, Gibert P, Gauthier D, Fromont E, Jullien JM, HARS J, 2005: Sondage sur les maladies abortives chez les ongulés sauvages et domestiques en alpages. Enquête menée dans la RNCFS des Bauges. Gibier Faune Sauvage 268, 24-32.
Juengel JL, McIntush EW, Niswender GD, 1999: Corpus luteum. In: Knobil E, Neill JD (eds), Encyclopedia of Reproduction. Academic Press, California; London, pp. 703-709.
Kanyari PWN, Munyua WK, Wilson AJ, 1986: Goat trypanosomiasis: trypanotolerance and epidemiology among goat breeds in Kenya. Bull Anim Health Prod Afr 34, 93-97.
Liggins GC, Thorburn GD, 1994: Initiation of parturition. In: Lamming GE (ed.), Pregnancy and Lactation. Marshall's Physiology of Reproduction, 4th edn. Chapman and Hall, London, 3, pp. 930-938.
Mori Y, Kano Y, 1984: Changes in plasma concentrations of LH, Progesterone and Oestradiol in relation to the occurrence of luteolysis, oestrus and time of ovulation in the shiba goat (Capra hircus). J Reprod Fert 72, 223-230.
Mourad M, 1995: Estimation of repeatability of litter size of Common African goats and cross with Alpine in Rwanda. Small Rum Res 19, 263-266.
Nagel RJ, Xie S, Roberts RM, 1993: Aspartic proteinases as markers of trophoblast differentiation in sheep. Biol Reprod 48(Suppl. 1), 39. (Abstract).
Ogaa JS, Kamar KK, Gombe S, Njogu AR, 1993: Abortion in goats following experimental infection with Trypanosoma congolense (abstract). In: Olaho-Mukani W, Munyua WK (eds), Proceedings International Scientific Council for Trypanosomiasis Research and Control. 21st Meeting of ISCTRC/OAU. Yamoussoukro, Ivory Coast, 176 p.
Osaer S, Goossens B, Kora S, Gaye M, Darboe L, 1999: Health and productivity of traditionally managed Djallonke sheep and West African Dwarf goats under high and moderate trypanosomosis risk. Vet Parasitol 82, 101-119.
Ranilla MJ, Sulon J, Carro MD, Mantecon AR, Beckers JF, 1994: ring gestation in Churra and Merino sheep. Theriogenology 42, 537-545.
Ranilla MJ, Sulon J, Carro MD, Mantecon AR, Beckers JF, Carro MD, 1997: Plasma pregnancy-associated glycoprotein and progesterone concentrations in pregnant Assaf ewes carrying single and twin lambs. Small Rum Res 24, 125-131.
Sawada T, Nakatani T, Tamada H, Mori J, 1994: Secretion of progesterone and 20 alpha-dihydroprogesterone during pregnancy in goats. Steroids 59, 468-471.
Sousa NM, Garbayo JM, Figueiredo JR, Sulon J, Gonçalves PBD, Beckers JF, 1999: Pregnancy-associated glycoprotein and progesterone profiles during pregnancy and postpartum in native goats from the north-east of Brazil. Small Rum Res 32, 137-147.
Statistical Analysis System (SAS), 1994: Statistics User's Guide, Version 6. SAS Institute Inc., Cary, NC.
Thorburn GD, Schneider W, 1972: The progesterone concentration in the plasma of the goat during the oestrus cycle and pregnancy. J Endocr 52, 23-36.
Vaitukaitis J, Robbins JB, Nieschlag E, Ross GT, 1971: A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol Metab 33, 988-991.
Vasques MI, Horta AEM, Marques CC, Sasser RG, Humblot P, 1995: Levels of bPSP-B throughout single and twin pregnancies after AI or transfer of IVM/IVF cattle embryos. Anim Reprod Sci 38, 279-289.
Waldeland H, Loken T, 1991: Reproductive failure in goats in Norway: an investigation in 24 herds. Acta Vet Scand 32, 535-541.
Wango EO, Wooding FBP, Heap RB, 1990: The role of trophoblastic binucleate cells in implantation in the goat: a quantitative study. Placenta 11, 381-394.
Wooding FBP, 1992: Current topic: the synepitheliochorial placenta of ruminants: binucleate cells fusions and hormone production. Placenta 13, 101-113.
Wooding FBP, Morgan G, Monaghan S, Hamon M, Heap RB, 1996: Functional specialization in the ruminant placenta: evidence for two populations of foetal binucleate cells of different selective synthetic capacity. Placenta 17, 75-86.
Wooding FBP, Roberts RM, Green JA, 2005: Light and electron microscope immunocytochemical studies of the distribution of pregnancy-associated glycoproteins (PAGs) throughout pregnancy in the cow: possible functional implications. Placenta 26, 807-827.
Xie S, Low BG, Nagel RJ, Kramer KK, Anthony RV, Zoli AP, Beckers JF, Roberts RM, 1991: Identification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family. Proc Natl Acad Sci USA 88, 10247-10251.
Xie S, Low BG, Nagel RJ, Beckers JF, Roberts RM, 1994: A novel glycoprotein of the aspartic proteinase gene family expressed in bovine placental trophectoderm. Biol Reprod 51, 1145-1153.
Xie S, Green JA, Bixby JB, Szafranska B, DeMartini JC, Hecht S, Roberts RM, 1997: The diversity and evolutionary relationships of the pregnancy-associated glycoproteins, as aspartic proteinase subfamily consisting of many trophoblast-expressed genes. Prod Natl Acad Sci USA 94, 12809-12816.
Zarrouk A, Engeland IV, Sulon J, Beckers JF, 1999a: Determination of pregnancy-associated glycoprotein concentrations in goats (Capra hircus) with unsuccessful pregnancies: a retrospective study. Theriogenology 51, 1321-1331.
Zarrouk A, Engeland IV, Sulon J, Beckers JF, 1999b: Pregnancy-associated glycoprotein levels in pregnant goats inoculated with Toxoplasma gondii or Listeria monocytogenes: a retrospective study. Theriogenology 52, 1095-1104.
Zoli AP, Beckers JF, Wouters-Ballman P, Closset J, Falmagne P, Ectors F, 1991: Purification and characterization of a bovine pregnancy-associated glycoprotein. Biol Reprod 45, 1-10.
Zoli AP, Guilbault LA, Delahaut P, Benitez-Ortiz W, Beckers JF, 1992: Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biol Reprod 46, 83-92.