Article (Scientific journals)
DNA polymorphism detection in Trifolium castaneum (Herbst) (Coleoptera: Tenebrionidae): potential use in stored product pest management
Braet, Y.; Haubruge, Eric; Gaspar, Charles et al.
1995In DNA Sequence: the Journal of DNA Sequencing and Mapping, 6 (1), p. 27-31
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Keywords :
Tribolium castaneum; Polymorphism; PCR; agricultural entomology; Animal; combination; DNA; DNA polymerase; enzymes; genetic markers; genetic polymorphism; genetics; insect pests; management; molecular genetics; Population; population genetics; stored products pests; strains
Abstract :
[en] Different pairs of oligonucleotide primers have used for the detection of DNA polymorphism between Tribolium castaneum strains. Amplification of a major DNA fragment and two minor was observed in all strains tested excepted for one strain from France which present only two major bands (respectively at 800 bp and 880 bp). Arbitrarily, the major band amplified from a DNA sample of the strain originating from the Philippines was choosen as a probe (sequence of 487 bp in length). The combination of this probe and restriction enzymes permitted the discrimination between strains. Our results show that RAP marker of Tribolium castaneum could be used to study its population genetics.
Disciplines :
Entomology & pest control
Author, co-author :
Braet, Y.
Haubruge, Eric  
Gaspar, Charles ;  Université de Liège - ULiège > Immunologie et vaccinologie
Burny, Arsène ;  Université de Liège - ULiège > Agro Biotech Gembloux
Language :
English
Title :
DNA polymorphism detection in Trifolium castaneum (Herbst) (Coleoptera: Tenebrionidae): potential use in stored product pest management
Publication date :
1995
Journal title :
DNA Sequence: the Journal of DNA Sequencing and Mapping
ISSN :
1042-5179
eISSN :
1029-2365
Publisher :
Taylor & Francis
Volume :
6
Issue :
1
Pages :
27-31
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 January 2018

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