Article (Scientific journals)
Purification and characterization of a carboxylesterase involved in malathion-specific resistance from Tribolium castaneum (Coleoptera: Tenebrionidae)
Haubruge, Eric; Amichot, Marcel; Cuany, André et al.
2002In Insect Biochemistry and Molecular Biology, 32 (9), p. 1181-1191
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Abstract :
[en] Specific resistance to malathion in a strain of Tribolium castaneum is due to a 44-fold increase in malathion carboxylesterase (MCE) activity relative to a susceptible strain, whereas non-specific esterase levels are slightly lower. Unlike the overproduced esterase of some mosquito and aphid species, MCE in Tribolium castaneum accounts for only a small fraction (0.033-0.045%) of the total extractable protein respectively in resistant and susceptible strains. The enzyme was purified to apparent homogeneity from these two strains and has a similar molecular weight of 62,000. However, preparative isoelectricfocusing indicated that resistant insects possess one MCE with pI of 7.3, while susceptible insects possess a MCE with a pI of 6.6. Purified MCE from both populations had different Km and Vm values for hydrolysis of malathion as well as for α-naphthyl acetate. The kinetic analysis suggests that MCE of resistant insects hydrolyses malathion faster than the purified carboxylesterase from susceptible beetles and that this enzyme has greater affinity for malathion than for naphthyl esters. Malathion-specific resistance is due to the presence of a qualitatively different esterase in the resistant strain.
Disciplines :
Entomology & pest control
Author, co-author :
Haubruge, Eric  
Amichot, Marcel
Cuany, André
Berge, Jean-Baptiste
Arnaud, Ludovic
Language :
English
Title :
Purification and characterization of a carboxylesterase involved in malathion-specific resistance from Tribolium castaneum (Coleoptera: Tenebrionidae)
Publication date :
2002
Journal title :
Insect Biochemistry and Molecular Biology
ISSN :
0965-1748
eISSN :
1879-0240
Publisher :
Elsevier Science
Volume :
32
Issue :
9
Pages :
1181-1191
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 January 2018

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